|本期目录/Table of Contents|

[1]公维丽,韩庆晔,马耀宏,等.滴定法快速测定发酵液中植酸酶活性[J].生物加工过程,2020,18(06):1-8.
 Gong Weili,Han Qingye,MA Yaohong,et al.A direct titration method for a rapid determination of phytase activity in fermentation broth[J].Chinese Journal of Bioprocess Engineering,2020,18(06):1-8.
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滴定法快速测定发酵液中植酸酶活性()
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
18
期数:
2020年06期
页码:
1-8
栏目:
出版日期:
2020-11-30

文章信息/Info

Title:
A direct titration method for a rapid determination of phytase activity in fermentation broth
作者:
公维丽12韩庆晔12马耀宏12王丙莲12杨 艳12孟庆军12杨俊慧12刘庆艾12蔡 雷12史建国12
1.齐鲁工业大学(山东省科学院),山东省科学院生物研究所
2.山东省生物传感器重点实验室
Author(s):
Gong Weili12 Han Qingye12 MA Yaohong12 Wang Binglian12 Yang Yan12 Meng Qingjun12 Yang Junhui12 Liu Qingai12 Cai Lei12 Shi Jianguo12
(1. Biology Institute of Shandong Academy of SciencesQilu University of Technology ( Shandong Academy of Sciences) , Jinan 250103, China; 2. Key Laboratory for Biosensors of Shandong ProvinceJinan 250103, China)
关键词:
植酸酶滴定植酸钠线性相关
分类号:
Q-31
摘要:
目前植酸酶发酵液中植酸酶活测定方法主要采用比色法,但此方法受到发酵液中磷的严重干扰,大大降低了检测的准确性。为避免传统方法中磷对检测过程的影响,本研究中,笔者基于酶活性可用单位时间内、单位体积中底物的减少量来表示的原理,通过自动滴定检测植酸钠浓度变化并建立分析模型计算出植酸酶活性。滴定结果显示:在200~1 000 U/mL范围内,不同酶活单位的标准植酸酶酶解底物前后滴定时间差值与酶活单位大小成正相关(R2=0.9905);此外,利用滴定法和国标法同时测定植酸酶酶活性,两者呈明显线性相关(R2=0.9973)。本研究建立的植酸酶活性测定方法耗时短、简单、易操作,对工业生产中植酸酶活性快速、准确测定具有重要意义。
Abstract:
Currently, the direct colorimetric assay for phytase activity was seriously interfered with the inorganic orthophosphate in phytase fermentation samples, resulting in large variation and poor reproducibility for phytase activity determination. To overcome this problem, based on the principle that the phytase activity could be defined as the decrease amount of substrate per unit time and volume, we established a direct titration method by automatically titrating the sodium phytate and establishing analytical model to calculate the phytase activity in fermentation broth in this study. The results demonstrated that a linear correlationship between titration time and standard phytase activity in the range of 200 U/mL-1 000 U/mL was observed (R2=0.9905), in addition, the phytase activity determined with this method was linearly dependent to that determined with national standard method (R2=0.9973). In conclusion, the direct titration method was time-saving, simple and user-friendly, which makes it possible for rapid and accurate phytase activity determination in large scale fermentation industry

参考文献/References:

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[7] 胡骁飞, 魏凤仙, 杨继飞, 等. 应用免疫学技术测定植酸酶活性的设想与展望[J].畜牧兽医学报,2015, 46(10):1697-1705.
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[9] 杨俊慧,公维丽,杨艳,等. 植酸酶工程菌产酶条件优化研究[J].山东科学, 2018, 31(4):79-84.Bae H D , Kj Y L C , Selinger L B . A novel staining method for detecting phytase activity[J]. J Microbiol Methods, 1999, 39(1):17-22.

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备注/Memo

备注/Memo:
基金项目:山东省科学院青年科学基金(2019QN002);山东省重点研发计划(2016ZDJS07A20)收稿日期:2019-05-07 修回日期:2019-09-29
作者简介:公维丽(1988-),女,山东临沂人,博士,助理研究员.研究方向:生物传感器用酶分子改造;史建国(联系人),研究员,E-mail:shijg1999@163.com
更新日期/Last Update: 2020-10-31