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[1]李谦,胡富,宁利敏,等.褐藻胶裂解酶的结构及催化机制研究进展[J].生物加工过程,2020,18(05):592-598.[doi:10.3969/j.issn.1672-3678.2020.05.008]
 LI Qian,HU Fu,NING Limin,et al.Insight into the structure and catalytic mechanism of alginate lyases[J].Chinese Journal of Bioprocess Engineering,2020,18(05):592-598.[doi:10.3969/j.issn.1672-3678.2020.05.008]
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褐藻胶裂解酶的结构及催化机制研究进展()
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
18
期数:
2020年05期
页码:
592-598
栏目:
出版日期:
2020-10-30

文章信息/Info

Title:
Insight into the structure and catalytic mechanism of alginate lyases
文章编号:
1672-3678(2020)05-0592-07
作者:
李谦12胡富2宁利敏3朱本伟2倪芳2姚忠2
1. 南京工业大学 生物与制药工程学院,江苏 南京 211800; 2. 南京工业大学 食品与轻工学院,江苏 南京 211800; 3. 南京中医药大学 医学与生命科学学院,江苏 南京 210023
Author(s):
LI Qian12HU Fu2NING Limin3ZHU Benwei2NI Fang2YAO Zhong2
1. College of Biotechnology and Pharmaceutical Engineering,Nanjing Tech University,Nanjing 211800,China; 2. College of Food Science and Light Industry,Nanjing Tech University,Nanjing 211800,China; 3. College of Medicine and Life Science,Nanjing University of Chinese Medicine,Nanjing 210023,China
关键词:
褐藻胶裂解酶 结构 催化机制 β果冻卷(α/α)n桶状结构 β螺旋
分类号:
Q557
DOI:
10.3969/j.issn.1672-3678.2020.05.008
文献标志码:
A
摘要:
褐藻胶是由β-D-甘露糖醛酸及其C5差向异构体α-L-古罗糖醛酸所组成的酸性多糖。褐藻胶裂解酶是多糖裂解酶的一种,可以将褐藻胶降解成寡糖或者单糖。近年来随着结构生物学的发展,越来越多褐藻胶裂解酶的结构及催化机制得到解析。本文中,笔者介绍了不同家族褐藻胶裂解酶结构以及催化机制的研究进展。根据酶序列来分,褐藻胶裂解酶分属到多糖裂解酶的7个家族; 从结构上来看,褐藻胶裂解酶通常采取3种结构:β果冻卷、(α/α)n桶状结构和β螺旋结构。褐藻胶裂解酶通过β消除的方式来降解褐藻胶,根据催化过程中有无金属离子的辅助,褐藻胶裂解酶的催化机制又可进一步分为His(Tyr)/Tyr型β消除和金属离子辅助的β消除。本文可以为褐藻胶裂解酶的应用提供一定的理论依据。
Abstract:
Alginate,a kind of linear acidic polysaccharide,consists of β-D-mannuronate and its C5 epimer α-L-guluronate.Alginate lyase belongs to the polysaccharide lyase(PL)family,it could degrade the alginate into oligosaccharides or monosaccharides. With the development in the technology of structure biology,more and more structural information and catalytic mechanism of alginate lyase has been determined in recent years.In this review,we summarized the recent progress of structure and catalytic mechanism of alginate lyases from different polysaccharide lyase families.The alginate lyase can be grouped into seven PL families based on the sequence similarity. However,the alginate lyase can be divided into three structures,namely the β-sheets jelly roll,the (α/α)n barrel and β-helix. Alginate lyase degrade the alginate by β-elimination. According to assistance of metal ions,the mechanism could further classified into three different ways-His(Tyr)/Tyr β type elimination,Tyr/Tyr β elimination and metal ion-assisted β elimination.This comprehensive information should be helpful regarding the application of alginate lyases.

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备注/Memo

备注/Memo:
收稿日期:2018-10-30修回日期:2018-12-22
基金项目:国家自然科学基金(31601410、81503463); 农业部海藻类肥料重点实验室开放基金(MAKLSF1808)
作者简介:李谦(1994—),男,江苏南京人,硕士研究生,研究方向:酶工程; 朱本伟(联系人),讲师,E-mail:zhubenwei@njtech.edu.cn; 宁利敏(联系人),副教授,E-mail:ninglimin@njucm.edu.cn
引文格式:李谦,胡富,宁利敏,等.褐藻胶裂解酶的结构及催化机制研究进展[J].生物加工过程,2020,18(5):592-598.
LI Qian,HU Fu,NING Limin,et al.Insight into the structure and catalytic mechanism of alginate lyases[J].Chin J Bioprocess Eng,2020,18(5):592-598..
更新日期/Last Update: 2020-09-30