|本期目录/Table of Contents|

[1]王宁,杨泽林,吴剑荣,等.鲫鱼籽唾液酸糖蛋白的N-糖链结构及生物活性[J].生物加工过程,2020,18(05):577-582.[doi:10.3969/j.issn.1672-3678.2020.05.006]
 WANG Ning,YANG Zelin,WU Jianrong,et al.N-Glycan structure and bioactivity of sialoglycoprotein from crucian carp eggs[J].Chinese Journal of Bioprocess Engineering,2020,18(05):577-582.[doi:10.3969/j.issn.1672-3678.2020.05.006]
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鲫鱼籽唾液酸糖蛋白的N-糖链结构及生物活性()
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
18
期数:
2020年05期
页码:
577-582
栏目:
出版日期:
2020-10-30

文章信息/Info

Title:
N-Glycan structure and bioactivity of sialoglycoprotein from crucian carp eggs
文章编号:
1672-3678(2020)05-0577-06
作者:
王宁杨泽林吴剑荣詹晓北
江南大学 糖化学与生物技术教育部重点实验室,江苏 无锡 214122
Author(s):
WANG NingYANG ZelinWU JianrongZHAN Xiaobei
Key Laboratory of Carbohydrate Chemistry and Biotechnology of the the Ministry of Education,Jiangnan University,Wuxi 214122,China
关键词:
鲫鱼籽 唾液酸化糖蛋白 N-糖链 结构分析 生物活性
分类号:
Q539
DOI:
10.3969/j.issn.1672-3678.2020.05.006
文献标志码:
A
摘要:
鲫鱼是常规食用淡水鱼,其鱼籽含有大量唾液酸化糖蛋白。对鲫鱼籽糖蛋白进行纯化,研究其N-糖链结构和生物活性。结果发现:鲫鱼籽糖蛋白分子量主要分布在19.7×104、2.7×104和2.2×104左右。利用基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)解析主要糖蛋白上N-糖链,发现其N-糖链结构主要为双天线型或三天线型分叉结构,末端唾液酸结合在半乳糖或N-乙酰氨基-半乳糖上。生物活性分析表明,鲫鱼籽糖蛋白具有阻止磷酸钙沉淀能力和一定清除DPPH自由基能力,对HaCat细胞具有促生长能力,并具有较强的促凝血活性。
Abstract:
Crucian carp(Carassius auratus)is a common freshwater fish in Chinese kitchen,and crucian carp egg contains a lot of sialoglycoproteins(CcSGP).In this work,the purification of SGP from crucian carp eggs,N-glycan structure and biological activity of CcSGP are investigated.Results show that CcSGP are rich in sialic acid,and the molecular weight distribution includes glycoprotein of 19.7×104,2.7×104 and 2.2×104.N-glycans analysis of CcSGP by MALDI-TOF mass spectrometry indicates that most of the N-glycan structure consists of double-antenna or tri-antenna structure,and the terminal sialic acid is bound to galactose or N-acetylgalactosamine.Bioactivity analysis indicates that CcSGP has the ability to prevent calcium phosphate precipitation and certain free radicals scavenging capacity of DPPH.Also,CcSGP has growth-promoting ability for HaCat cells and distinct procoagulant activity.

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备注/Memo

备注/Memo:
收稿日期:2019-02-03修回日期:2020-05-27
基金项目:国家重点研发计划(2017YFD0400302)
作者简介:王宁(1991—),男,河南驻马店人,硕士研究生,研究方向:天然活性产物; 吴剑荣(联系人),副教授,E-mail:kinowu@jiangnan.edu.cn
引文格式:王宁,杨泽林,吴剑荣,等.鲫鱼籽唾液酸糖蛋白的N-糖链结构及生物活性[J].生物加工过程,2020,18(5):577-582.
WANG Ning,YANG Zelin,WU Jianrong,et al.N-Glycan structure and bioactivity of sialoglycoprotein from crucian carp eggs[J].Chin J Bioprocess Eng,2020,18(5):577-582..
更新日期/Last Update: 2020-09-30