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[1]袁琪,陶惟一,羊亚平,等.利用具有过水解活性的乙酰基木聚糖酯酶催化制备过氧乙酸[J].生物加工过程,2020,18(05):567-571.[doi:10.3969/j.issn.1672-3678.2020.05.004]
 YUAN Qi,TAO Weiyi,YANG Yaping,et al.Production of peracetic acid by acetyl xylan esterase with perhydrolysis activity[J].Chinese Journal of Bioprocess Engineering,2020,18(05):567-571.[doi:10.3969/j.issn.1672-3678.2020.05.004]
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利用具有过水解活性的乙酰基木聚糖酯酶催化制备过氧乙酸()
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
18
期数:
2020年05期
页码:
567-571
栏目:
出版日期:
2020-10-30

文章信息/Info

Title:
Production of peracetic acid by acetyl xylan esterase with perhydrolysis activity
文章编号:
1672-3678(2020)05-0567-05
作者:
袁琪1陶惟一1羊亚平2李霜1
1.南京工业大学 生物与制药工程学院,江苏 南京 211800; 2.江苏亚邦强生药业有限公司,江苏 金坛 213100
Author(s):
YUAN Qi1TAO Weiyi1YANG Yaping2LI Shuang1
1. College of Biotechnology and Pharmaceutical Engineering,Nanjing Tech University,Nanjing 211800,China 2. Jiangsu Yabang Qiangsheng Pharmaceutical Co.,Ltd.,Jintan 213100,China
关键词:
过氧乙酸 乙酰木聚糖酯酶 固定化酶 过水解活性
分类号:
Q81
DOI:
10.3969/j.issn.1672-3678.2020.05.004
文献标志码:
A
摘要:
以甘油三乙酸酯和H2O2为底物,利用来源枯草芽孢杆菌的乙酰木聚糖酯酶(AXE)游离酶和固定化酶催化过水解反应制备过氧乙酸(PAA),并以化学法制备的过氧乙酸为对照组进行性质比较。结果表明:游离酶用量(酶蛋白)为1.0 g/L,可得到11.6 g/L的过氧乙酸,对甘油三乙酸酯的转化率为16.7%; 游离酶用量(酶蛋白)为75 mg/L,过氧乙酸质量浓度可达3.5 g/L,对甘油三乙酸酯的转化率为15.3%; 固定化酶用量(树脂量)为50 g/L,可得3.6 g/L的过氧乙酸。低浓度过氧乙酸比高浓度过氧乙酸更稳定。将对照组和酶法制备过氧乙酸的质量浓度均设置为3.5 g/L,其pH分别为1.98和4.45; 对照组使铜片形成重度腐蚀,对碳钢有轻度腐蚀性,对不锈钢和铝基本无腐蚀; 酶法制备的过氧乙酸对上述4种金属均无腐蚀。
Abstract:
Peracetic acid(PAA)was obtained by free and immobilized acetyl xylan esterase(AXE)of Bacillus subtilis, with glyceryl triacetate and hydrogen peroxide as substrate.The results showed that in the presence of 1.0 g/L crude AXE(protein concentration),11.6 g/L PAA could be obtained,the percent conversion of glyceryl triacetate was 16.7%.In the presence of 75 mg/L crude AXE,3.5 g/L PAA could be obtained,the percent conversion of glyceryl triacetate was 15.3%.In the presence of 50 g/L immobilized AXE,3.6 g/L PAA could be obtained.The low concentration products were more stable than the high concentration ones.When chemocatalysis prepared and the enzymatic prepared PAAs were set to concentration as 3.5 g/L,the pH was 1.98 and 4.45,respectively.In the chemocatalysis prepared PAA,severe corrosion and mild corrosion were found on the copper and carbon steel,respectively.The AXE prepared PAA exhibited no corrosion activity toward the two materials.

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备注/Memo

备注/Memo:
收稿日期:2018-07-18修回日期:2020-01-09
基金项目:江苏省先进生物制造创新中心自主研究课题(XTD1824)
作者简介:袁琪(1994—),女,江苏张家港人,硕士研究生,研究方向:微生物学; 李霜(联系人),教授,E-mail:lishuang@njtech.edu.cn
引文格式:袁琪,陶惟一,羊亚平,等.利用具有过水解活性的乙酰基木聚糖酯酶催化制备过氧乙酸[J].生物加工过程,2020,18(5):567-571.
YUAN Qi,TAO Weiyi,YANG Yaping,et al.Production of peracetic acid by acetyl xylan esterase with perhydrolysis activity[J].Chin J Bioprocess Eng,2020,18(5):567-571..
更新日期/Last Update: 2020-09-30