|本期目录/Table of Contents|

[1]王薛雪,李莎,任战坤,等.γ-聚谷氨酸的抗氧化及对UV辐射致皮肤细胞光老化的修复作用[J].生物加工过程,2020,18(05):561-566.[doi:10.3969/j.issn.1672-3678.2020.05.003]
 WANG Xuexue,LI Sha,REN Zhankun,et al.Antioxidation of poly-(γ-glutamic acid)and repair of photoaging fibroblasts induced by UV radiation[J].Chinese Journal of Bioprocess Engineering,2020,18(05):561-566.[doi:10.3969/j.issn.1672-3678.2020.05.003]
点击复制

γ-聚谷氨酸的抗氧化及对UV辐射致皮肤细胞光老化的修复作用()
分享到:

《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
18
期数:
2020年05期
页码:
561-566
栏目:
出版日期:
2020-10-30

文章信息/Info

Title:
Antioxidation of poly-(γ-glutamic acid)and repair of photoaging fibroblasts induced by UV radiation
文章编号:
1672-3678(2020)05-0561-06
作者:
王薛雪1李莎1任战坤2王瑞1詹伊婧1符婵2徐虹1
1. 南京工业大学 食品与轻工学院,江苏 南京 211800; 2. 南京轩凯生物科技有限公司,江苏 南京 211800
Author(s):
WANG Xuexue1LI Sha1REN Zhankun2WANG Rui1ZHAN Yijing1FU Chan2XU Hong1
1.College of Food and Light Industry,Nanjing Tech University,Nanjing 211800,China; 2.Nanjing Shineking Biotech Co.,Ltd.,Nanjing 211800,China
关键词:
γ-聚谷氨酸 分子量 自由基 光老化修复 抗氧化 抗衰老 化妆品
分类号:
Q599
DOI:
10.3969/j.issn.1672-3678.2020.05.003
文献标志码:
A
摘要:
寻找天然抗氧化物用于修复皮肤光老化问题是目前的研究热点之一。γ-聚谷氨酸(γ-PGA)是由微生物发酵制备的一种天然高分子聚合物材料,具有清除自由基的能力,但其抗氧化、抗皮肤光老化效果及与γ-PGA分子量的关系尚未明确。本文中,笔者考察不同分子量的γ-PGA对超氧阴离子自由基(·O-2)、羟基自由基(·OH)和1,1-二苯基-2-三硝基苯肼(·DPPH)的清除能力。结果显示:不同分子量的γ-PGA对以上3种自由基均表现出一定程度的自由基清除活性,清除率可分别达31.36%、97.55%和74.64%。通过构建紫外线诱导小鼠皮肤成纤维细胞(L929)氧化损伤光老化模型,确定不同分子量的γ-PGA都能一定程度修复光老化细胞损伤并能减少光老化细胞中氧自由基(ROS)、氮自由基(NO)的增加,其中分子量3.0×105γ-PGA修复光老化细胞损伤的效果最佳。
Abstract:
Finding natural antioxidants for reparing skin photoaging is one of the research focuses at present. Poly-γ-glutamic acid(γ-PGA)is a natural polymer material prepared by microbial fermentation. γ-PGA has the ability of scavenging free radicals, but the relationship between the antioxidative effect, skin photoaging effect and molecular weight of γ-PGA has not been clarified in this paper, the scavenging ability of γ-PGA with different molecular weights on superoxide anion free radical,hydroxyl free radical and DPPH free radical was investigated.γ-PGA with different molecular weights exhibited scavenging activity on the above three free radicals with the scavenging rate of 31.36%,97.55% and 74.64%,respectively.In addition,by constructing ultraviolet-induced photoaging models in L929,γ-PGA with different molecular weights could repair photoaging cell damage and reduce ROS,NO in photoaging cells.γ-PGA with molecular weight 3.0×105 γ-PGA had the best effect in repairing photoaging cell damage.

参考文献/References:

[1] KAMMEYER A,LUITEN R M.Oxidation events and skin aging[J].Ageing Res Rev,2015,21:16-29.
[2] POON F,KANG S,CHIEN A L.Mechanisms and treatments of photoaging[J].Photodermatol Photoimmunol Photomed,2015,31(2):65-74.
[3] 严芳,卢寅.皮肤光老化研究进展[J].亚太传统医药,2011,7(11):183-185.
[4] TRAUTINGER F.Mechanisms of photodamage of the skin and its functional consequences forskinaging[J].Clin Exp Dermatol,2001,26(7):573-577.
[5] IANNACONE M R,HUGHES M C B,GREENA C.Effects of sunscreen on skin cancer and photoaging[J].Photodermatol Photoimmunol Photomed,2014,30(2/3):55-61.
[6] EL GHISSASSI F,BAAN R,STRAIF K,et al.A review of human carcinogens,part D:radiation[J].Lancet Oncol,2009,10(8):751-752.
[7] 宋菲,王挥,唐敏敏,等.天然椰子油提取物对H2O2诱导的HSF 细胞氧化损伤的保护作用[J].日用化学工业,2017,47(9):517-521.
[8] 李晓晓,李刚,何玉凤,等.高分子抗氧化剂研究进展[J].化学通报,2011,74(4):316-324.
[9] LIU X,LIU F,LIU S,et al.Poly-γ-glutamate from Bacillus subtilis inhibits tyrosinase activity and melanogenesis[J].Appl Microbiol Biotechnol,2013,97(22):9801-9809.
[10] SHIH I L,VAN Y T.The production of poly-(γ-glutamic acid)from microorganisms and its various applications[J].Bioresour Technol,2001,79(3):207-225.
[11] 张天娇,刘霞,邓观杰,等.γ-聚谷氨酸体外抗氧化性及抑制透明质酸酶活性的研究[J].食品与药品,2017,19(3):153-157.
[12] MARKLUND S,MARKLUND G.Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase[J].Eur J Biochem,1974,47:469-474.
[13] LIU W,XU J,JING P,et al.Preparation of a hydroxypropyl Ganodermalucidum polysaccharide and its physicochemical properties[J].Food Chem,2010,122(40):965-971.
[14] ZENG Q H,ZHANG X W,XU X L,et al.Antioxidant and anticomplement functions of flavonoids extracted from Penthorum chinense Pursh[J].Food Function,2013,4(12):1811-1818.
[15] 陈明亮.UVA致人成纤维细胞和角质形成细胞损伤机制及白藜芦醇的光保护作用[D].长沙:中南大学,2008.
[16] LEE J,CHO H,CHOI J,et al.Chemical sporulation and germination:cytoprotective nanocoating of individual mammalian cells with a degradable tannic acid-Fe-III complex[J].Nanoscale,2015,7(45):18918-18922.
[17] 刘扬,金光辉,刘树铮.流式细胞术在紫外线照射后细胞内活性氧检测中的应用[J].吉林大学学报(医学版),2004(6):975-977.
[18] 李新.氨基酸和酚类化合物的联合抗氧化作用研究[D].长春:吉林大学,2017.
[19] CHOE E,MIN D B.Mechanisms of antioxidants in the oxidation of foods[J].Compr Rev Food Sci Food Safety,2009,8(4):345-358.
[20] SUETSUNA K,UKEDA H,OCHI H.Isolation and characterization of free radical scavenging activities peptides derived from casein[J].J Nutr Biochem,2000,11(3):128-131.

相似文献/References:

[1]冯志彬,李昆,石秀峰,等.混菌发酵生产γ-聚谷氨酸的工艺条件优化[J].生物加工过程,2012,10(05):23.[doi:10.3969/j.issn.1672-3678.2012.05.005]
 FENG Zhibin,LI Kun,SHI Xiufeng,et al.Optimization of culture conditions for producing poly(γ-glutamic acid)by mixed fermentation[J].Chinese Journal of Bioprocess Engineering,2012,10(05):23.[doi:10.3969/j.issn.1672-3678.2012.05.005]

备注/Memo

备注/Memo:
收稿日期:2018-08-26修回日期:2018-10-09
基金项目:国家自然科学基金(51703095)
作者简介:王薛雪(1994—),女,江苏无锡人,硕士研究生,研究方向:生物化工; 徐虹(联系人),教授,E-mail:xuh@njtech.edu.cn
引文格式:王薛雪,李莎,任战坤,等.γ-聚谷氨酸的抗氧化及对UV辐射致皮肤细胞光老化的修复作用[J].生物加工过程,2020,18(5):561-566.
WANG Xuexue,LI Sha,REN Zhankun,et al.Antioxidation of poly-(γ-glutamic acid)and repair of photoaging fibroblasts induced by UV radiation[J].Chin J Bioprocess Eng,2020,18(5):561-566..
更新日期/Last Update: 2020-09-30