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[1]徐彤,郑旭东,崔羽歆,等.新疆沙枣叶提取物对春尺蠖多酚氧化酶的抑制作用[J].生物加工过程,2020,18(03):340-345.[doi:10.3969/j.issn.1672-3678.2020.03.011]
 XU Tong,ZHENG Xudong,CUI Yuxin,et al.Inhibitory effects of two compounds extracted from leaves of Elaeagnus oxycarpa Schlechtend in Xinjiang on polyphenol oxidase of larva Apochemia cinerarius Ersch[J].Chinese Journal of Bioprocess Engineering,2020,18(03):340-345.[doi:10.3969/j.issn.1672-3678.2020.03.011]
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新疆沙枣叶提取物对春尺蠖多酚氧化酶的抑制作用()
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
18
期数:
2020年03期
页码:
340-345
栏目:
出版日期:
2020-05-30

文章信息/Info

Title:
Inhibitory effects of two compounds extracted from leaves of Elaeagnus oxycarpa Schlechtend in Xinjiang on polyphenol oxidase of larva Apochemia cinerarius Ersch
文章编号:
1672-3678(2020)03-0340-06
作者:
徐彤郑旭东崔羽歆王俊朱慧玲付建红姚雪丹
新疆师范大学 生命科学学院 新疆特殊环境物种多样性应用与调控重点实验室新疆特殊环境物种保护与调控生物学实验室,新疆 乌鲁木齐 830054
Author(s):
XU TongZHENG XudongCUI YuxinWANG JunZHU HuilingFU JianhongYAO Xuedan
Xinjiang Key Laboratory of Special Species Conservation and Regulatory Biology, Key Laboratory of Species Diversity Application and Control in Xinjiang,College of Life Science, Xinjiang Normal University,Urumqi 830054,China
关键词:
尖果沙枣 春尺蠖 多酚氧化酶 酶学性质 抑制剂
分类号:
Q554
DOI:
10.3969/j.issn.1672-3678.2020.03.011
文献标志码:
A
摘要:
多酚氧化酶在昆虫的变态发育和免疫防御中起着非常重要的作用,可作为害虫控制的一个重要作用靶标。采用40%饱和度硫酸铵沉淀和Sephadex G-100凝胶过滤等步骤,对产自于乌鲁木齐的春尺蠖幼虫多酚氧化酶进行分离,并研究其酶学性质。结果表明:多酚氧化酶的比酶活为617.21 U/mg,是粗酶液的6.06倍; 该酶对焦性没食子酸、邻苯二酚和L-多巴的Km值分别为6.45、1.11和1.3 mmol/L; 在pH 6.5、25 ℃时,多酚氧化酶活性最高; 当温度高于45 ℃时,随着保温时间的延长,酶活力下降明显。采用中压色谱和C18反相制备型色谱技术对尖果沙枣叶乙醇提取物进行分离,得到6个化合物,鉴定了其中2个化合物; 将这2个化合物用于多酚氧化酶的抑制实验,结果表明分离到的化合物6和化合物7均显示出抑制活性,IC50值约为0.125 mg/mL。
Abstract:
Polyphenol oxidase plays important roles in metamorphosis development and immune defense of insects,then it can be as an important target against the pest control.The polyphenol oxidase(PO)from Apochemia cinerarius Ersch larvae in Urumqi was partially purified by 40% saturated(NH4)2SO4 and sephadex G-100 gel filtration and enzymatic properties were studied.The results showed that the specific activity of PO was 617.21 U/mg and PO was purified 6.06-fold of the crude enzyme.The kinetic parameters for the oxidation of pyrogallic acid,catechol and L-dopamine(L-DOPA)by the PO were 6.45,1.11 and 1.3 mmol/L,respectively.The optimum pH was 6.5 and the best temperature was 25 ℃ for the tested PO.The enzyme activity decreased obviously with the prolongation of holding time above 45 ℃.By medium pressure chromatography(MPLC)and C18-reversed phase chromatography,6 compounds were isolated from the ethanol extracts of Elaeagnus oxycarpa Schlechtend leaves,2 of which were identified.The inhibition of PO showed that the isolated compounds 6 and 7 showed inhibition activity and the inhibitor concentrations of leading to 50% activity lost(IC50)were estimated to be 0.125 mg/mL for the two compounds.

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备注/Memo

备注/Memo:
收稿日期:2020-03-10修回日期:2020-03-18
基金项目:新疆维吾尔自治区自然科学基金(2016D01A054)
作者简介:徐彤(1995—),女,黑龙江大庆人,硕士研究生,研究方向:天然活性产物的研究与开发; 付建红(联系人),教授,E-mail:fjh_719@163.com
引文格式:徐彤,郑旭东,崔羽歆,等.新疆沙枣叶提取物对春尺蠖多酚氧化酶的抑制作用[J].生物加工过程,2020,18(3):340-345.
XU Tong,ZHENG Xudong,CUI Yuxin,et al.Inhibitory effects of two compounds extracted from leaves of Elaeagnus oxycarpa Schlechtend in Xinjiang on polyphenol oxidase of larva Apochemia cinerarius Ersch[J].Chin J Bioprocess Eng,2020,18(3):340-345..
更新日期/Last Update: 2020-05-30