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 WANG Huiyang,QIN Jing,ZHUANG Dan,et al.Isolation,characterization,antioxidant and immunomodulating activity of polysaccharides from Achatina fulica mucus[J].Chinese Journal of Bioprocess Engineering,2020,18(03):324-331.[doi:10.3969/j.issn.1672-3678.2020.03.009]





Isolation,characterization,antioxidant and immunomodulating activity of polysaccharides from Achatina fulica mucus
1.南京工业大学 生物与制药工程学院,江苏 南京 211800; 2.南京工业大学 药学院,江苏 南京 211800; 3.中国中医科学院 中药资源中心,北京 100700
WANG Huiyang1QIN Jing2ZHUANG Dan2LIU Chunyao2ZHANG Yi1ZHANG Lihui1L? Guangping 23
1.School of Biotechnology and Pharmaceutical Engineering,Nanjing Tech University,Nanjing 211800,China; 2.School of Pharmaceutical Sciences,Nanjing Tech University,Nanjing 211800,China; 3.National Resource Center for Chinese Materia Medica,China Academy of
蜗牛黏液 多糖 结构表征 抗氧化 免疫调节活性
利用高效凝胶色谱串联紫外、示差和多角度激光散射检测器(HPSEC-UV-RI-MALLS )对蜗牛黏液中的多糖进行分子量分布表征。利用气相色谱串联质谱(GC-MS)和红外光谱(FT-IR)对其化学结构进行表征; 并对所获得的多糖进行抗氧化及免疫活性的评价。结果显示:蜗牛黏液中主要含有5个组分的多糖,是其重要的活性成分。对这5个多糖进行鉴定,其平均分子量为4.549×106、1.392×105、6.291×104、5.262×104 和4.153×104 。单糖由岩藻糖、甘露糖、葡萄糖和半乳糖组成,摩尔比为1.69:2.46:0.12:1。多糖主链为→2)Manp-(1→,→3)Fucp-(1→和→3)Manp-(1→。此外,蜗牛黏液多糖具有明显的抗氧化作用,能很好地清除ABTS·+和·OH,IC50值分别为2.35和4.70 mg/mL; 且能明显增强巨噬细胞(RAW264.7)的吞噬能力,促进NO和白细胞介素(IL-6)、肿瘤坏死因子(TNF-α)等免疫细胞因子的释放。
Polysaccharide is one of important bioactive components in Achatina fulica mucus.In this study, the polysaccharides in A. fulica mucus were isolated,and the high performance size exclusion chromatography coupled to ultraviolet detector-refractive index detector-multi-angle laser light scattering(HPSEC-UV-RI-MALLS)was developed to evaluate the molecular weights distribution.The chemical structure including monosaccharide composition and glycosidic linkage were characterized by gas chromatography mass spectrometry(GC-MS).The results show that there mainly contained 5 polysaccharides, and they were important active components. We identified the structures of the five polysaccharides, and the average molecular weights were 4.549×106,1.392×105,6.291×104,5.262×104 and 4.153×104. The monosaccharides were of fucose,mannose,glucose,and galactose in the molar ratio of 1.69:2.46:0.12:1. And the backbone of the most abundant polysaccharide was composed by →2)Manp-(1→,→3)Fucp-(1→ and →3)Manp-(1→. Furthermore,the A. fulica mucus polysaccharides exhibited significantly antioxidant effect. They could remove ABTS·+ and hydroxyl radicals well, and the half-inhibitory concentration was 2.35 and 4.70 mg/mL. The in vitro experiments show that it could enhance the ability of devouring FITC-dextran of peritoneal macrophages and promote the release of nitric oxide and immune reactive molecules such as interleukin(IL)-6 and tumor necrosis factor(TNF)-α.


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基金项目:国家自然科学基金青年基金(81603290); 江苏省自然科学基金青年基金(BK20170988); 国家重点研发计划(2018YFC1603400)
作者简介:王慧阳(1993—),女,陕西渭南人,硕士研究生,研究方向:生物工程; 吕广萍(联系人),副教授,E-mail:lvguangping@njtech.edu.cn
WANG Huiyang,QIN Jing,ZHUANG Dan,et al.Isolation,characterization,antioxidant and immunomodulating activity of polysaccharides from Achatina fulica mucus[J].Chin J Bioprocess Eng,2020,18(3):324-331..
更新日期/Last Update: 2020-05-30