|本期目录/Table of Contents|

[1]沈瑞华,郭军玲,周哲敏.腈水合酶的稳定性改造研究进展[J].生物加工过程,2020,18(03):269-276.[doi:10.3969/j.issn.1672-3678.2020.03.001]
 SHEN Ruihua,GUO Junling,ZHOU Zhemin.Recent advances in stability reconstruction of nitrile hydratase[J].Chinese Journal of Bioprocess Engineering,2020,18(03):269-276.[doi:10.3969/j.issn.1672-3678.2020.03.001]
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腈水合酶的稳定性改造研究进展()
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
18
期数:
2020年03期
页码:
269-276
栏目:
出版日期:
2020-05-30

文章信息/Info

Title:
Recent advances in stability reconstruction of nitrile hydratase
文章编号:
1672-3678(2020)03-0269-08
作者:
沈瑞华1郭军玲2周哲敏2
1.江苏昌九农科化工有限公司,江苏 南通 226413; 2.江南大学 生物工程学院,江苏 无锡 214122
Author(s):
SHEN Ruihua1GUO Junling2ZHOU Zhemin2
1. Jiangsu Changjiu Agrochemical Co.,Ltd.,Nantong 226413,China; 2.School of Biotechnology,Jiangnan University,Wuxi 214122,China
关键词:
腈水合酶 生物催化 稳定性改造 蛋白质工程 工业应用
分类号:
Q789
DOI:
10.3969/j.issn.1672-3678.2020.03.001
文献标志码:
A
摘要:
腈水合酶(nitrile hydratase,NHase,EC 4.2.1.84)是一种金属酶,能催化腈类物质转化为相应的酰胺类物质,广泛应用于高附加值酰胺类化合物的生产。但由于NHase较差的稳定性,在催化反应过程中受到温度、产物浓度等影响而逐渐失活,这严重影响了NHase的工业化应用。近年来,针对这个问题,研究者通过酶分子改造,在NHase的稳定性提高方面取得了一定进展。本文中,笔者主要对近年来提高NHase稳定性的分子改造方面取得的进展进行总结,包括基于生物信息学解析的盐桥增设、自由能降低、同源片段重组和基因融合等策略。这些成果不仅推动了生物催化法生产酰胺类化合物的发展,还将为NHase蛋白质改造工程提供理论支持。
Abstract:
Nitrile hydratase(NHase,EC 4.2.1.84)is a key metalloenzyme in the hydration of nitriles to their corresponding amides that are widely used in the industrial production of highly value amides.However,the poor thermostability of NHase is the major factor preventing its extensive industrial application.We summarize here the molecular modification methods to improve the stability of NHase,including salt bridge insertion,free energy reduction,homologous fragment recombination,and gene fusion.These achievements not only improve the development of biocatalytic production of amide compounds,but also provide theoretical support for NHase protein engineering.

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备注/Memo

备注/Memo:
收稿日期:2020-03-25修回日期:2020-04-22
基金项目:国家自然科学基金(21878125)
作者简介:沈瑞华(1970—),男,江苏如皋人,工程师,研究方向:发酵工程; 周哲敏(联系人),教授,E-mail:zhmzhou@jiangnan.edu.cn
引文格式:沈瑞华,郭军玲,周哲敏.腈水合酶的稳定性改造研究进展[J].生物加工过程,2020,18(3):269-276.
SHEN Ruihua,GUO Junling,ZHOU Zhemin.Recent advances in stability reconstruction of nitrile hydratase[J].Chin J Bioprocess Eng,2020,18(3):269-276..
更新日期/Last Update: 2020-05-30