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[1]杨亚茹,李婷婷,励建荣.基于群体感应的海鲈鱼源杀鲑气单胞菌CS12与水产特定腐败菌的共培养[J].生物加工过程,2020,18(02):256-262.[doi:10.3969/j.issn.1672-3678.2020.02.015]
 YANG Yaru,LI Tingting,LI Jianrong.Co-culture of Aeromonas salmonicida CS12 in sea bass and specific spoilage organism of aquatic products based on quorum sensing[J].Chinese Journal of Bioprocess Engineering,2020,18(02):256-262.[doi:10.3969/j.issn.1672-3678.2020.02.015]
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基于群体感应的海鲈鱼源杀鲑气单胞菌CS12与水产特定腐败菌的共培养()
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
18
期数:
2020年02期
页码:
256-262
栏目:
出版日期:
2020-03-30

文章信息/Info

Title:
Co-culture of Aeromonas salmonicida CS12 in sea bass and specific spoilage organism of aquatic products based on quorum sensing
文章编号:
1672-3678(2020)02-0256-07
作者:
杨亚茹1李婷婷2励建荣1
1.渤海大学 食品科学与工程学院 生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心 辽宁省食品安全重点实验室 辽宁省高等学校生鲜食品产业技术研究院,辽宁 锦州 121013; 2.大连民族大学 生命科学学院,辽宁 大连 116600
Author(s):
YANG Yaru1LI Tingting2LI Jianrong1
1.The Fresh Food Storage and Processing Technology Research Institute of Liaoning Provincial Universities, Food Safety Key Laboratory of Liaoning Province,National & Local Joint Engineering Research Center of Storage,Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products,College of Food Science and Technology,Bohai University, Jinzhou 121013,China; 2.College of Life Science,Dalian Minzu University,Dalian 116600,China
关键词:
海鲈鱼 杀鲑气单胞菌 群体感应 共培养 抑制作用
分类号:
TS254.9
DOI:
10.3969/j.issn.1672-3678.2020.02.015
文献标志码:
A
摘要:
杀鲑气单胞菌在水产品致腐、致病等方面存在潜在危害,为探究其危害机制及可能的控制方法,笔者基于群体感应系统对杀鲑气单胞菌自身生长、产信号分子能力以及与水产品常见特定腐败菌共培养情况进行了研究。结果表明:从腐败海鲈鱼中分离鉴定得到一株杀鲑气单胞菌CS12,与杀鲑气单胞菌B52的16S rDNA序列相似度达99%; 该菌株不耐酸,pH≤5.0不生长; 能够诱导紫色杆菌CV026产紫色且紫圈扩散明显,气质检测分泌信号分子主要为C6-HSL; 比较各单菌及共培养的情况发现,杀鲑气单胞菌CS12与不同腐败菌共培养时在不同生长时期表现出不同的生长特征; 与荧光假单胞菌PF03共培养时存在群体感应抑制现象,产信号分子能力明显减弱。表明杀鲑气单胞菌CS12与荧光假单胞菌PF03共培养产生了较好的群体感应抑制效果。
Abstract:
Aeromonas salmonicida is a potential hazard that cause spoilage and disease in aquatic products.In order to explore its hazard mechanism and control methods,the growth,quorum signals,and co-culture characteristics of A.salmonicida CS12,isolated from spoiled sea bass,were studied and identified.The results indicate that A.salmonicida CS12 could not grow below pH 5.0. Strain CS12 mainly produced C6-HSL of signaling molecules with density-dependent,induced Chromobacterium violaceum CV026 to produce purple color,and the purple circle had obvious diffusion.Co-culturing the strain showed different growth characteristics at different growth stages.Its co-culture had no effect on the growth rate during exponential phase.When co-cultured with Pseudomonas fluorescens,the bacterial density and the activity of AHLswas significantly inhibited.These results show that P.fluorescens had a great inhibitory activity in co-culture.

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备注/Memo

备注/Memo:
收稿日期:2019-11-11修回日期:2019-12-10
基金项目:国家自然科学基金(31471639); 国家重点研发计划(2017YFD0400106、2018YFD0400600、2018LNSPLM0104)
作者简介:杨亚茹(1994—),女,陕西渭南人,硕士研究生,研究方向:水产品贮藏加工及质量安全控制; 励建荣(联系人),教授,E-mail:lijr6491@163.com; 李婷婷(联系人),副教授,E-mail:jwltt@dlnu.edu.cn
引文格式:杨亚茹,李婷婷,励建荣.基于群体感应的海鲈鱼源杀鲑气单胞菌CS12与水产特定腐败菌的共培养[J].生物加工过程,2020,18(2):256-262.
YANG Yaru,LI Tingting,LI Jianrong.Co-culture of Aeromonas salmonicida CS12 in sea bass and specific spoilage organism of aquatic products based on quorum sensing[J].Chin J Bioprocess Eng,2020,18(2):256-262..
更新日期/Last Update: 2020-03-31