|本期目录/Table of Contents|

[1]杨威,严立石,刘航,等.表面活性剂协同离子液体促进纤维素原位酶解[J].生物加工过程,2019,17(06):562-568.[doi:10.3969/j.issn.1672-3678.2019.06.003]
 YANG Wei,YAN Lishi,LIU Hang,et al.Synergistic effects of surfactants and ionic liquid on promoting in situ hydrolysis of cellulose[J].Chinese Journal of Bioprocess Engineering,2019,17(06):562-568.[doi:10.3969/j.issn.1672-3678.2019.06.003]
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表面活性剂协同离子液体促进纤维素原位酶解()
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
17
期数:
2019年06期
页码:
562-568
栏目:
出版日期:
2019-11-30

文章信息/Info

Title:
Synergistic effects of surfactants and ionic liquid on promoting in situ hydrolysis of cellulose
文章编号:
1672-3678(2019)06-0562-07
作者:
杨威严立石刘航胡翠英扶教龙鞠鑫李良智
苏州科技大学 化学生物与材料工程学院,江苏 苏州 215009
Author(s):
YANG WeiYAN LishiLIU HangHU CuiyingFU JiaolongJU XinLI Liangzhi
School of Chemistry,Biology and Material Engineering,Suzhou University of Science and Technology,Suzhou 215009,China
关键词:
离子液体 纤维素酶 表面活性剂 酶动力学 原位酶解 生物质资源
分类号:
TQ35
DOI:
10.3969/j.issn.1672-3678.2019.06.003
文献标志码:
A
摘要:
研究表面活性剂对在离子液体(IL)1-乙基-3-甲基咪唑磷酸二乙酯盐([Emim]DEP)中纤维素酶的稳定性影响,并通过动态光散射(DLS)、荧光光谱、圆二色谱(CD)以及酶动力学考察在IL体系中,表面活性剂与纤维素酶相互作用的机制。DLS结果显示,阴、阳离子表面活性剂分别使纤维素酶的zeta电位处于较低的负电荷区域(-19.2 mV)以及正电荷区域(10 mV)。荧光光谱和CD分析结果表明,阴、阳离子表面活性剂对纤维素酶的二级、三级结构影响较大。动力学结果表明表面活性剂对纤维素酶活性的增强,主要表现为米氏常数Km值变小。使用1 g/L Tween 80协同50 g/L[Emim]DEP原位酶解甘蔗渣纤维素,转化率提高了9.2%,一定程度上提高了原料的利用率,可为纤维素酶解工艺提供技术参考,为生物质资源的综合利用提供理论依据。

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备注/Memo

备注/Memo:
收稿日期:2019-05-10修回日期:2019-10-09
基金项目:国家自然科学基金(21676173); 苏州市产业技术创新专项(农业科技创新)(SNG201611); 江苏高校青蓝工程
作者简介:杨威(1993—),男,江苏淮安人,硕士研究生,研究方向:生物催化转化; 李良智(联系人),教授,硕士生导师,E-mail:liliangzhi01@163.com
引文格式:杨威,严立石,刘航,等.表面活性剂协同离子液体促进纤维素原位酶解[J].生物加工过程,2019,17(6):562-568.
YANG Wei,YAN Lishi,LIU Hang,et al.Synergistic effects of surfactants and ionic liquid on promoting in situ hydrolysis of cellulose[J].Chin J Bioprocess Eng,2019,17(6):562-568..
更新日期/Last Update: 2019-11-30