|本期目录/Table of Contents|

[1]朱五二,缪明永,顾正华,等.盐单胞菌S62 β-半乳糖苷酶合成低聚半乳糖的研究[J].生物加工过程,2019,17(02):131-137.[doi:10.3969/j.issn.1672-3678.2019.02.003]
 ZHU Wuer,MIAO Mingyong,GU Zhenghua,et al.Synthesis of galactooligosaccharides by β-galactosidase from Halomonas sp. S62[J].Chinese Journal of Bioprocess Engineering,2019,17(02):131-137.[doi:10.3969/j.issn.1672-3678.2019.02.003]
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盐单胞菌S62 β-半乳糖苷酶合成低聚半乳糖的研究()
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
17
期数:
2019年02期
页码:
131-137
栏目:
出版日期:
2019-03-30

文章信息/Info

Title:
Synthesis of galactooligosaccharides by β-galactosidase from Halomonas sp. S62
文章编号:
1672-3678(2019)02-0131-07
作者:
朱五二12缪明永3顾正华12李由然2丁重阳12石贵阳2
1. 江南大学 生物工程学院 糖化学与生物技术教育部重点实验室,江苏 无锡 214122; 2. 江南大学 粮食发酵工艺与技术国家工程实验室,江苏 无锡 214122; 3. 第二军医大学 生物化学与分子生物学教研室,上海 200433
Author(s):
ZHU Wuer12MIAO Mingyong3GU Zhenghua12LI Youran2DING Zhongyang12SHI Guiyang2
1. Key Laboratory of Carbohydrate Chemistry and Biotechnology of the Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China; 2. National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University,Wuxi 214122,China; 3. Department of Biochemistry and Molecular Biology, Second Military Medical University,Shanghai 200433,China
关键词:
β-半乳糖苷酶 低聚半乳糖产率 乳糖消耗率 盐单胞菌
分类号:
TS219
DOI:
10.3969/j.issn.1672-3678.2019.02.003
文献标志码:
A
摘要:
以盐单胞菌S62 β-半乳糖苷酶为研究对象,探究其合成低聚半乳糖效率。为了提高低聚半乳糖产率,对反应条件进行了优化,并以反应温度、pH、加酶量、底物浓度为考察对象进行正交试验,得到最优反应条件:反应温度40 ℃,pH 7.0,加酶量50 U/mL,底物质量浓度300 g/L。反应6 h时可获得最大低聚半乳糖产率(41.91±0.27)%,乳糖消耗率为(82.47±0.38)%。反应4~8 h内低聚半乳糖产率都维持在40%以上,此时乳糖消耗率均在80%以上,在提高乳糖利用率的同时实现了低聚半乳糖的高产,有利于降低生产成本,为低温S62 β-半乳糖苷酶工业化应用奠定了基础。

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相似文献/References:

[1]邢肖肖,齐崴,王梦凡,等.β-半乳糖苷酶酶学性质及其在低聚半乳糖合成中的应用[J].生物加工过程,2015,13(02):30.[doi:10.3969/j.issn.1672-3678.2015.02.006]
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备注/Memo

备注/Memo:
收稿日期:2018-03-22修回日期:2018-05-28
基金项目:国家高技术研究发展计划(863计划)(2014AA093513); 江苏省科技项目(BN2016202)
作者简介:朱五二(1992—),男,安徽安庆人,硕士研究生,研究方向:发酵工学; 丁重阳(联系人),教授,E-mail:zyding@jiangnan.edu.cn
引文格式:朱五二,缪明永,顾正华,等.盐单胞菌S62 β-半乳糖苷酶合成低聚半乳糖的研究[J].生物加工过程,2019,17(2):131-137.
ZHU Wuer,MIAO Mingyong,GU Zhenghua,et al.Synthesis of galactooligosaccharides by β-galactosidase from Halomonas sp. S62[J].Chin J Bioprocess Eng,2019,17(2):131-137..
更新日期/Last Update: 2019-03-30