|本期目录/Table of Contents|

[1]程功,焦思明,贾培媛,等.毕赤酵母表达环状芽胞杆菌壳聚糖酶及其水解产物组成与结构分析[J].生物加工过程,2019,17(01):110-116.[doi:10.3969/j.issn.1672-3678.2019.01.015]
 CHENG Gong,JIAO Siming,JIA Peiyuan,et al.Expression of chitosanase from Bacillus circulans in Pichia pastoris and analysis of its hydrolysates[J].Chinese Journal of Bioprocess Engineering,2019,17(01):110-116.[doi:10.3969/j.issn.1672-3678.2019.01.015]
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毕赤酵母表达环状芽胞杆菌壳聚糖酶及其水解产物组成与结构分析()
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
17
期数:
2019年01期
页码:
110-116
栏目:
出版日期:
2019-01-30

文章信息/Info

Title:
Expression of chitosanase from Bacillus circulans in Pichia pastoris and analysis of its hydrolysates
文章编号:
1672-3678(2019)01-0110-07
作者:
程功焦思明贾培媛任立世冯翠张毓宸李建军杜昱光
中国科学院 过程工程研究所 生化工程国家重点实验室,北京 100190
Author(s):
CHENG GongJIAO SimingJIA PeiyuanREN LishiFENG CuiZHANG YuchenLI JianjunDU Yuguang
State Key Laboratory of Biochemical Engineering,Institute of Process Engineering, Chinese Academy of Sciences,Beijing 100190,China
关键词:
环状芽胞杆菌 壳聚糖酶 毕赤酵母 壳寡糖 超高效液相色谱-四极杆飞行时间质谱 核磁共振
分类号:
Q819
DOI:
10.3969/j.issn.1672-3678.2019.01.015
文献标志码:
A
摘要:
壳寡糖具有抗炎、抗肿瘤及激活植物免疫等一系列生物活性,这些生物活性的发挥与其结构直接相关。为最大程度发挥壳寡糖的生物活性并确定其结构与功能关系,有必要制备不同结构特征的壳寡糖。笔者优化并全基因合成环状芽胞杆菌壳聚糖酶基因,利用毕赤酵母进行分泌表达; 用该酶水解制备低脱乙酰度壳寡糖,使用超高效液相色谱-四极杆飞行时间质谱对其组分进行分离及鉴定,使用核磁共振确定其末端结构特征。结果表明:所得壳聚糖酶蛋白质量浓度达0.77 mg/mL,水解产物中包含至少37种不同的壳寡糖组分,这些组分的还原末端主要由氨基葡萄糖组成,非还原末端同时包含氨基葡萄糖及N-乙酰氨基葡萄糖。本研究为壳寡糖结构与功能关系的阐释奠定基础。

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备注/Memo

备注/Memo:
收稿日期:2018-06-29修回日期:2018-07-12
基金项目:国家重点研发计划(2017YFD0200902); 国家自然科学基金联合基金(U1608255); 中国科学院重点部署项目(KFZD-SW-218)
作者简介:程功(1984—),男,安徽合肥人,博士,助理研究员,研究方向:糖生物工程; 杜昱光(联系人),研究员,E-mail:ygdu@ipe.ac.cn
引文格式:程功,焦思明,贾培媛,等.毕赤酵母表达环状芽胞杆菌壳聚糖酶及其水解产物组成与结构分析[J].生物加工过程,2019,17(1):110-116.
CHENG Gong,JIAO Siming,JIA Peiyuan,et al.Expression of chitosanase from Bacillus circulans in Pichia pastoris and analysis of its hydrolysates [J].Chin J Bioprocess Eng,2019,17(1):110-116..
更新日期/Last Update: 2019-01-30