|本期目录/Table of Contents|

[1]张建芬,柯薇,陈虹.葡萄糖3-脱氢酶工程菌的构建、表达及其合成3-酮对硝基苯井冈霉胺[J].生物加工过程,2019,17(01):94-98.[doi:10.3969/j.issn.1672-3678.2019.01.012]
 ZHANG Jianfeng,KE Wei,CHEN Hong.Construction and expression of a glucoside 3-dehydrogenase engineering strain for N-p-nitrophenyl-3-ketovalidamine biosynthesis[J].Chinese Journal of Bioprocess Engineering,2019,17(01):94-98.[doi:10.3969/j.issn.1672-3678.2019.01.012]
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葡萄糖3-脱氢酶工程菌的构建、表达及其合成3-酮对硝基苯井冈霉胺()
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
17
期数:
2019年01期
页码:
94-98
栏目:
出版日期:
2019-01-30

文章信息/Info

Title:
Construction and expression of a glucoside 3-dehydrogenase engineering strain for N-p-nitrophenyl-3-ketovalidamine biosynthesis
文章编号:
1672-3678(2019)01-0094-05
作者:
张建芬柯薇陈虹
浙江树人大学 生物与环境工程学院,浙江 杭州 310015
Author(s):
ZHANG JianfengKE WeiCHEN Hong
College of Biology and Environmental Engineering,Zhejiang Shuren University,Hangzhou 310015,China
关键词:
葡萄糖3-脱氢酶 基因挖掘 3-酮对硝基苯井冈霉胺 细胞转化
分类号:
Q78
DOI:
10.3969/j.issn.1672-3678.2019.01.012
文献标志码:
A
摘要:
采用基因挖掘技术,从NCBI数据库发现来源于Glaciecola polaris的葡萄糖3-脱氢酶基因,该基因经密码子优化后合成,构建pET28b-GpG3DH重组表达载体,转入大肠杆菌E.coli BL21中进行重组表达,结果显示:GpG3DH实现了部分可溶表达,其分子量为5.5×104。随后,优化了GpG3DH的表达条件,结果发现,重组菌在TB培养基中37 ℃培养至OD600为0.8时,加入IPTG至终浓度为0.5 mmol/L,16 ℃诱导表达10 h,GpG3DH酶活为2.83×10-2 U/mL。进一步将重组菌用于3-酮对硝基苯井冈霉胺的细胞转化法合成,细胞用10 mmol/L的EDTA处理,在pH为7.5、25 ℃的条件下转化32 h,3-酮对硝基苯井冈霉胺的产率(Y3-KpNPV)为0.54。

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2018-05-15修回日期:2018-05-24
基金项目:国家自然科学基金青年基金(21102131); 浙江省自然科学基金(LY18C010002)
作者简介:张建芬(1979—),女,浙江杭州人,博士,教授,研究方向:生物催化与生物转化,E-mail:grace_zjf@126.com
引文格式:张建芬,柯薇,陈虹.葡萄糖3-脱氢酶工程菌的构建、表达及其合成3-酮对硝基苯井冈霉胺[J].生物加工过程,2019,17(1):94-98.
ZHANG Jianfeng,KE Wei,CHEN Hong.Construction and expression of a glucoside 3-dehydrogenase engineering strain for N-p-nitrophenyl-3-ketovalidamine biosynthesis[J].Chin J Bioprocess Eng,2019,17(1):94-98..
更新日期/Last Update: 2019-01-30