|本期目录/Table of Contents|

 CHEN Peipei,GONG Linjun,L? Yongqin.New development and application of porous monoliths as solid supports for immobilization of enzymes[J].Chinese Journal of Bioprocess Engineering,2019,17(01):36-43.[doi:10.3969/j.issn.1672-3678.2019.01.006]





New development and application of porous monoliths as solid supports for immobilization of enzymes
北京化工大学 生命科学与技术学院 北京市生物加工过程重点实验室,北京 100029
CHEN PeipeiGONG LinjunL? Yongqin
Beijing Key Laboratory of Bioprocess,Life Science and Technology,Beijing University of Chemical Technology,Beijing 100029,China
整体柱 固定化酶 纳米材料 固定化方法


[1] PARTHASARATHY R V,MARTIN C R.Synthesis of polymeric microcapsule arrays and their use for enzyme immobilization[J].Nature,1994,369:298-301.
[2] 白云岫,曹逊,戈钧.高分子修饰无机晶体固定化酶研究进展[J].生物加工过程,2018,16(1):1672-3678.
[3] MOULD D L,SYNGE R L M.Electrokinetic ultrafiltration analysis of polysaccharides:a new approach to the chromatography of large molecules[J].Analyst,1952,77:964-969.
[4] KUB?N M,PACˇEK P,CHROMECˇEK R.Gel permeation chromatography on porous poly(ethylene glycol methacrylate)[J].Chem Commun,1967,32(11):3881-3887.
[5] HJERT?N S,LIAO J L,ZHANG R.High-performance liquid chromatography on continuous polymer beds[J].J Chromatogr A,1989,473(1):273-275.
[6] SVEC F,FR?CHET J M J.Continuous rods of macroporous polymer as high performance liquid chromatography separation media[J].Anal Chem,1992,64(7):820-822.
[7] ISHIZUKA N,KOBAYASHI H,MINAKUCHI H.Monolithic silica columns for high-efficiency separations by high-performance liquid chromatography[J].J Chromatogr A,2002,960(1/2):85-96.
[8] HE P,GREENWAY G,HASWELL S J.Development of a monolith based immobilized lipase micro-reactor for biocatalytic reactions in a biphasic mobile system[J].Process Biochem,2010,45(4):593-597.
[9] ANUAR S T,MUGO S M,CURTIS J M.A flow-through enzymatic microreactor for the rapid conversion of triacylglycerols into fatty acid ethyl ester and fatty acid methyl ester derivatives for GC analysis[J].Anal Methods,2015,7(14):5898-5906.
[10] KAWAKAMI K,UENO M,TAKEI T,et al.Application of a Burkholderia cepacia lipase-immobilized silica monolith micro-bioreactor to continuous-flow kinetic resolution for transesterification of(R,S)-1-phenylethanol[J].Process Biochem,2012,47(1):147-150.
[11] SZYMANSKA K,PUDO W,MROWIEC-BIAON J,et al.Immobilization of invertase on silica monoliths with hierarchical pore structure to obtain continuous flow enzymatic microreactors of high performance[J].Micropor Mesopor Mater,2013,170:75-82.
[12] HE P,DAVIES J,GREENWAY G,et al.Measurement of acetylcholinesterase inhibition using bienzymes immobilized monolith micro-reactor with integrated electrochemical detection[J].Anal Chim Acta,2010,659(1/2):9-14.
[13] YESIL-CELIKTAS O,CUMANA S,SMIRNOVA I.Silica-based monoliths for enzyme catalyzed reactions in microfluidic systems with an emphasis on glucose 6-phosphate dehydrogenase and cellulase[J].Chem Eng J,2013,234:166-172.
[14] PIROZZI D,ABAGNALE M,MINIERI L,et al.In-situ sol-gel modification strategies to develop a monolith continuous microreactor for enzymatic green reactions[J].Chem Eng J,2016,306:1010-1016.
[15] CHEN S,WEN L,SVEC F,et al.Magnetic metal-organic frameworks as scaffolds for spatial co-location and positional assembly of multi-enzyme systems enabling enhanced cascade biocatalysis[J].RSC Adv,2017,7(34):21205-21213.
[16] YIN J,CHEN S,ZHANG N,et al.Multienzyme cascade bioreactor for a 10 min digestion of genomic DNA into single nucleosides and quantitative detection of structural DNA modifications in cellular genomic DNA[J].ACS Appl Mater Interfaces,2018,10(26):21883-21890.
[17] RIVERA J G,MESSERSMITH P B.Polydopamine-assisted immobilization of trypsin onto monolithic structures for protein digestion[J].J Sep Sci,2012,35(12):1514-1520.
[18] FOO H C,SMITH N W,STANLEY S M.Fabrication of an on-line enzyme micro-reactor coupled to liquid chromatography-tandem mass spectrometry for the digestion of recombinant human erythropoietin[J].Talanta,2015,135:18-22.
[19] 吕永琴.新型功能化整体柱的制备、应用和分离识别机理的研究[D].北京:北京化工大学,2010.
[20] XIE S,SVEC F,FRECHET J M J.Design of reactive porous polymer supports for high throughput bioreactors:poly(2-vinyl-4,4-dimethylazlactone-co-acrylamide-co-ethyl dimethacrylate[J].Biotechnol Bioeng,1999,62(1):30-35.
[21] YAO C,QI L,HU W,et al.Immobilization of trypsin on sub-micron skeletal polymer monolith[J].Anal Chim Acta,2011,692(1/2):131-137.
[22] OZONER S K,KESKINLER B,ERHAN E.HRP immobilized microporous poly(styrene-divinylbenzene-polyglutaraldehyde)monolith for forced flow injected phenol biosensing[J].Mater Sci Eng C,2011,31(3):663-668.
[23] JONNADA M,EL RASSI Z.Poly(N-acryloxysuccinimide-co-ethylene glycol dimethacrylate)precursor monolith and its post polymerization modification with alkyl ligands,trypsin and lectins for reversed-phase chromatography,miniaturized enzyme reactors and lectin affinity chromatography,respectively[J].Electrophoresis,2017,38(22/23):2870-2879.
[24] WU S,ZHANG L,YANG K,et al.Preparing a metal-ion chelated immobilized enzyme reactor based on the polyacrylamide monolith grafted with polyethylenimine for a facile regeneration and high throughput tryptic digestion in proteomics[J].Anal Bioanal Chem,2012,402(2):703-710.
[25] KRENKOVA J,LACHER N A,SVEC F.Highly Efficientenzyme reactors containing trypsin and endoproteinase LysC immobilized on porous polymer monolith coupled to MS suitable for analysis of antibodies[J].Anal Chem,2009,81:2004-2012.
[26] SUN X,XIN Y,WANG X,et al.Functionalized acetoacetylated poly(vinyl alcohol)monoliths for enzyme immobilization:a phase separation method[J].Colloid Polym Sci,2017,295(10):1827-1833.
[27] UYAMA H,WANG G.Reactive poly(ethylene-co-vinyl alcohol)monoliths with tunable pore morphology for enzyme immobilization[J].Colloid Polym Sci,2015,293(8):2429-2435.
[28] KOLB H C,FINN M G,SHARPLESS K B.Click chemistry:diverse chemical function from a few good reactions[J].Angew Chem Int Ed,2001,40:2004-2021.
[29] CELEBI B,BAYRAKTAR A,TUNCEL A.Synthesis of a monolithic,micro-immobilised enzyme reactor via click-chemistry[J].Anal Bioanal Chem,2012,403(9):2655-2663.
[30] WU M,ZHANG H,WANG Z,et al."One-pot" fabrication of clickable monoliths for enzyme reactors[J].Chem Commun(Camb),2013,49(14):1407-1409.
[31] LAFLEUR J P,SENKBEIL S,NOVOTNY J,et al.Rapid and simple preparation of thiol-ene emulsion-templated monoliths and their application as enzymatic microreactors[J].Lab Chip,2015,15(10):2162-2172.
[32] JONSSON A,SVEJDAL R R,BOGELUND N,et al.Thiol-enemonolithic pepsin microreactor with a 3D-printed interface for efficient UPLC-MS peptide mapping analyses[J].Anal Chem,2017,89(8):4573-4580.
[33] LI N,ZHENG W,SHEN Y,et al.Preparation of a novel polymer monolith with functional polymer brushes by two-step atom-transfer radical polymerization for trypsin immobilization[J].J Sep Sci,2014,37(23):3411-3417.
[34] XIONG M,GU B,ZHANG J D,et al.Glucose microfluidic biosensors based on reversible enzyme immobilization on photopatterned stimuli-responsive polymer[J].Biosens Bioelectron,2013,50:229-234.
[35] WU S,MA J,YANG K,et al.A novel organic-inorganic hybrid monolith for trypsin immobilization[J].Sci China Life Sci,2011,54(1):54-59.
[36] MA J,HOU C,LIANG Y,et al.Efficient proteolysis using a regenerable metal-ion chelate immobilized enzyme reactor supported on organic-inorganic hybrid silica monolith[J].Proteomics,2011,11(5):991-995.
[37] YUAN H,ZHANG L,ZHANG Y.Preparation of high efficiency and low carry-over immobilized enzymatic reactor with methacrylic acid-silica hybrid monolith as matrix for on-line protein digestion[J].J Chromatogr A,2014,1371:48-57.
[38] CHEN Y,WU M,WANG K,et al.Vinyl functionalized silica hybrid monolith-based trypsin microreactor for on line digestion and separation via thiol-ene "click" strategy[J].J Chromatogr A,2011,1218(44):7982-7988.
[39] ZHOU Y,WAN M M,GAO L,et al.One-pot synthesis of a hierarchical PMO monolith with superior performance in enzyme immobilization[J].J Mater Chem B,2013,1(12):1738-1748.
[40] ANSARI S A,HUSAIN Q.Potential applications of enzymes immobilized on/in nano materials:a review[J].Biotechnol Adv,2012,30(3):512-523.
[41] LV Y,ALEJANDRO F M,FRECHET J M,et al.Preparation of porous polymer monoliths featuring enhanced surface coverage with gold nanoparticles[J].J Chromatogr A,2012,1261:121-128.
[42] SHANGGUAN L,ZHANG L,XIONG Z,et al.Investigation of bi-enzymatic reactor based on hybrid monolith with nanoparticles embedded and its proteolytic characteristics[J].J Chromatogr A,2015,1388:158-166.
[43] XU S,MO R,JIN C,et al.Mesoporous silica nanoparticles incorporated hybrid monolithic stationary phase immobilized with pepsin for enantioseparation by capillary electrochromatography[J].J Pharm Biomed Anal,2017,140:190-198.
[44] HONG T,CHEN X,XU Y,et al.Preparation of graphene oxide-modified affinity capillary monoliths based on three types of amino donor for chiral separation and proteolysis[J].J Chromatogr A,2016,1456:249-256.


 BAI Yunxiu,CAO Xun,GE Jun.Advances in enzyme-polymer conjugates and enzyme-inorganic crystal composites[J].Chinese Journal of Bioprocess Engineering,2018,16(01):12.[doi:10.3969/j.issn.1672-3678.2018.01.002]


作者简介:陈佩佩(1993—),女,河北石家庄人,硕士研究生,研究方向:分离纯化; 吕永琴(联系人),教授,E-mail:lvyq@mail.buct.edu.cn
CHEN Peipei,GONG Linjun,Lü Yongqin.New development and application of porous monoliths as solid supports for immobilization of enzymes[J].Chin J Bioprocess Eng,2019,17(1):36-43..
更新日期/Last Update: 2019-01-30