|本期目录/Table of Contents|

[1]王亚军,陆颖,周艳芬,等.柠檬苦素对葡聚糖硫酸钠诱导的小鼠结肠炎的治疗作用及机制[J].生物加工过程,2018,16(06):57-62.[doi:10.3969/j.issn.1672-3678.2018.06.011]
 WANG Yajun,LU Ying,ZHOU Yanfeng,et al.Therapeutic effect and mechanism of limonin on dextran sulfate sodium salt-induced ulcerative colitis in mice[J].Chinese Journal of Bioprocess Engineering,2018,16(06):57-62.[doi:10.3969/j.issn.1672-3678.2018.06.011]
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柠檬苦素对葡聚糖硫酸钠诱导的小鼠结肠炎的治疗作用及机制()
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
16
期数:
2018年06期
页码:
57-62
栏目:
出版日期:
2018-11-30

文章信息/Info

Title:
Therapeutic effect and mechanism of limonin on dextran sulfate sodium salt-induced ulcerative colitis in mice
文章编号:
1672-3678(2018)06-0057-06
作者:
王亚军陆颖周艳芬王宇郭素菡张琪孙阳
南京工业大学 药学院,江苏 南京 211800
Author(s):
WANG YajunLU YingZHOU YanfengWANG YuGUO SuhanZHANG QiSUN Yang
School of Pharmaceutical Sciences,Nanjing Tech University,Nanjing 211800,China
关键词:
柠檬苦素 溃疡性结肠炎 葡聚糖硫酸钠 核转录因子kappa B(NF-κB) 生物医药
分类号:
R966
DOI:
10.3969/j.issn.1672-3678.2018.06.011
文献标志码:
A
摘要:
探讨柠檬苦素(limonin)对葡聚糖硫酸钠(dextran sulfate sodium salt,DSS)致实验性小鼠溃疡性结肠炎(ulcerative colitis,UC)模型的治疗作用及其机制。将20只8周龄雌性C57BL/6J小鼠,随机分为正常对照组、模型组、柠檬苦素组和5-氨基水杨酸组,每组5只。正常的小鼠每日给予蒸馏水饮用,其余各组小鼠给予30 g/L DSS连续饮用7 d后,继续饮用正常水3 d,诱导小鼠急性UC模型。药物治疗组小鼠分别灌胃给予柠檬苦素和5-氨基水杨酸的剂量为50和50 mg/(kg·d),每日1次,连续灌胃治疗10 d。实验终点时,分析实验动物的体质量变化、结肠长度、结肠组织病理学变化及细胞核转录因子kappa B(NF-κB)的蛋白表达。结果发现:与模型组比较,柠檬苦素可显著改善DSS诱导的小鼠急性结肠炎症状,包括体质量降低、结肠长度缩短、稀便和血便等,减轻小鼠急性结肠炎结肠组织的病理损伤,抑制炎症细胞的浸润和肠黏膜坏死。此外,柠檬苦素亦可以降低结肠炎小鼠结肠组织细胞核内NF-κB p65蛋白表达,抑制NF-κB p65的激活。柠檬苦素通过抑制NF-κB p65的激活,缓解DSS诱导的小鼠结肠炎症,发挥治疗作用。

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备注/Memo

备注/Memo:
收稿日期:2018-01-19修回日期:2018-05-31
基金项目:国家自然科学基金青年基金(81703556); 江苏省自然科学基金青年基金(BK20171024)
作者简介:王亚军(1986—),湖北荆州人,硕士研究生,研究方向:药理学; 孙阳(联系人),讲师,E-mail:sunyangsunny@sina.com
引文格式:王亚军,陆颖,周艳芬,等.柠檬苦素对葡聚糖硫酸钠诱导的小鼠结肠炎的治疗作用及机制[J].生物加工过程,2018,16(6):57-62.
WANG Yajun,LU Ying,ZHOU Yanfeng,et al.Therapeutic effect and mechanism of limonin on dextran sulfate sodium salt-induced ulcerative colitis in mice[J].Chin J Bioprocess Eng,2018,16(6):57-62..
更新日期/Last Update: 2018-11-30