|本期目录/Table of Contents|

[1]杨威,牛欢青,陈晓春,等.正十六烷对节杆菌发酵产环磷酸腺苷的影响[J].生物加工过程,2018,16(06):24-29.[doi:10.3969/j.issn.1672-3678.2018.06.005]
 YANG Wei,NIU Huanqing,CHEN Xiaochun,et al.Effects of n-hexadecane on the biosynthesis of cyclic adenosine monophosphate by Arthrobacter sp. CGMCC 3584[J].Chinese Journal of Bioprocess Engineering,2018,16(06):24-29.[doi:10.3969/j.issn.1672-3678.2018.06.005]
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正十六烷对节杆菌发酵产环磷酸腺苷的影响()
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
16
期数:
2018年06期
页码:
24-29
栏目:
出版日期:
2018-11-30

文章信息/Info

Title:
Effects of n-hexadecane on the biosynthesis of cyclic adenosine monophosphate by Arthrobacter sp. CGMCC 3584
文章编号:
1672-3678(2018)06-0024-06
作者:
杨威牛欢青陈晓春陈勇朱晨杰柳东庄伟应汉杰
南京工业大学 生物与制药工程学院 材料化学工程国家重点实验室 江苏先进生物与化学制造协同创新中心,江苏 南京 211800
Author(s):
YANG WeiNIU HuanqingCHEN XiaochunCHEN YongZHU ChenjieLIU DongZHUANG WeiYING Hanjie
Jiangsu National Synergetic Innovation Center for Advanced Materials,State Key Laboratory of Materials-Oriented Chemical Engineering,College of Biotechnology and Pharmaceutical Engineering,Nanjing Tech University,Nanjing 211800,China
关键词:
氧载体 正十六烷 节杆菌 环磷酸腺苷
分类号:
Q814
DOI:
10.3969/j.issn.1672-3678.2018.06.005
文献标志码:
A
摘要:
溶氧在节杆菌发酵产环磷酸腺苷(cAMP)过程中起着重要作用。本研究中,笔者首先对4种氧载体(正癸烷、正十二烷、正十四烷和正十六烷)的最佳添加浓度和添加时间进行筛选。结果表明:在0 h添加2%(体积分数)正十六烷促进cAMP生产效果最佳。在5 L 发酵罐中添加2%(体积分数)正十六烷后,细胞干质量(DCW)和cAMP产量分别达到10.85 g/L和8.87 g/L,比对照分别提高了19.4%和23.2%。氧载体的加入提高了发酵液中的氧传递系数(KLa),降低了胞内NADH/NAD+比率以及胞内三磷酸腺苷(ATP)水平,而对关键酶的酶活影响不显著。

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2017-08-01修回日期:2018-05-24
基金项目:国家重点基础研究发展计划(973计划)(2013CB733602); 国家高技术研究发展计划(863计划)(2012AA021203); 国家自然科学基金重大研究项目(21390204); 国家科技支撑计划(2012BAI44G01); 国家自然科学基金面上项目(2137611); 长江学者和创新团队项目(IRT_14R28); 国家自然科学基金青年项目(21506097、21606128); 江苏省高校优势学科建设工程(PAPD)
作者简介:杨威(1992—),男,陕西汉中人,硕士研究生,研究方向:生物工程; 应汉杰(联系人),教授,E-mail:yinghanjie@njtech.edu.cn; 牛欢青(联系人),助理研究员,E-mail:huanqingniu@126.com
引文格式:杨威,牛欢青,陈晓春,等.正十六烷对节杆菌发酵产环磷酸腺苷的影响[J].生物加工过程,2018,16(6):24-29.
YANG Wei,NIU Huanqing,CHEN Xiaochun,et al.Effects of n-hexadecane on the biosynthesis of cyclic adenosine monophosphate by Arthrobacter sp.CGMCC 3584[J].Chin J Bioprocess Eng,2018,16(6):24-29..
更新日期/Last Update: 2018-11-30