|本期目录/Table of Contents|

[1]韦元琪,钱旭,雍晓雨,等.一株耐盐酵母的分离、鉴定及其生物学特性[J].生物加工过程,2018,16(06):1-7.[doi:10.3969/j.issn.1672-3678.2018.06.001]
 WEI Yuanqi,QIAN Xu,YONG Xiaoyu,et al.Isolation,identification and characterization of a salt tolerant yeast[J].Chinese Journal of Bioprocess Engineering,2018,16(06):1-7.[doi:10.3969/j.issn.1672-3678.2018.06.001]
点击复制

一株耐盐酵母的分离、鉴定及其生物学特性()
分享到:

《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
16
期数:
2018年06期
页码:
1-7
栏目:
出版日期:
2018-11-30

文章信息/Info

Title:
Isolation,identification and characterization of a salt tolerant yeast
文章编号:
1672-3678(2018)06-0001-07
作者:
韦元琪1钱旭1雍晓雨12贾红华12周华1蔡恒1
1.南京工业大学 生物与制药工程学院,江苏 南京 211800; 2.南京工业大学 生物能源研究所,江苏 南京 211800
Author(s):
WEI Yuanqi1QIAN Xu1YONG Xiaoyu12JIA Honghua12ZHOU Hua1CAI Heng1
1.College of Biotechnology and Pharmaceutical Engineering,Nanjing Tech University,Nanjing 211800,China; 2. Bioenergy Research Institute,Nanjing Tech University,Nanjing 211800,China
关键词:
库德里阿兹威氏毕赤酵母 分离 鉴定 耐盐性 海藻糖 ATPase
分类号:
Q939.97
DOI:
10.3969/j.issn.1672-3678.2018.06.001
文献标志码:
A
摘要:
以洋河酒厂的酒曲为材料,首先利用盐浓度梯度富集培养和氯化三苯四唑(TTC)染色法初筛获得18株酵母菌株,再通过杜氏小管产气及摇瓶发酵实验,从18株初筛菌株中分离得到1株在1.4 mol/L NaCl浓度下生长性能较好的菌株,命名为W58。 经形态观察和26S rDNA基因序列比对分析,该酵母菌株被鉴定为1株库德里阿兹威氏毕赤酵母(Pichia kudriavzevii)。对菌株的生长性能及耐受性进行研究发现:该菌最适生长温度为30 ℃,最适pH为6.0,在高盐条件下(1.4 mol/L NaCl),其胞内海藻糖的积累显著增加,比对照菌株高28.6%。此外,菌株W58在高盐条件下,其ATPase活性比对照菌显著提高(约3倍)。综上所述,菌株W58在高盐条件下能通过胞内积累海藻糖以及维持一定的ATPase活性抵抗外界环境压力。

参考文献/References:

[1] 贺东海.耐高渗酒精酵母的筛选[D].济南:山东轻工业学院,2011.
[2] 侯丽华,宋茜,曹小红.酱油风味研究进展[J].中国酿造,2009,28(7):1-3.
[3] 毛志群,张伟,檀建新.高产酒精酵母的筛选及鉴定[J].食品与发酵工业,2002,29(3):50-53.
[4] ZHOU Y,YIN X C,DUAN R J,et al.SpAHA1 and SpSOS1 coordinate in transgenic yeast to improve salt tolerance[J].PLoS ONE,2015,10(9):e0137447.
[5] VAN DER SLUIS C,TRAMPER J,WIJFFELS R H.Enhancing and acceleration flavor formation by salt-tolerant yeasts in Japanese soy-sauce processes[J].Trends Food Sic Tech,2001,12(9):322-327.
[6] 谢韩,丁洪波.添加耐盐酵母改善低盐固态酱油风味[J].江苏调味副食品,2002(4):6-7.
[7] 卫永华,齐威,王春玲,等.耐盐酵母在酱油中的应用及其耐盐机理的研究[J].中国酿造,2012,31(2):25-28.
[8] RODRíGUEZ-NAVARRO A.Potassium transport in fungi and plants[J].Biochim Biophys Acta,2000,1469:1-30.
[9] GONZáLEZ-HERNáNDEZ J C,JIMéNEZ-ESTRADA M,PE?A A.Comparative analysis of trehalose production by Debaryomyces hansenii and Saccharomyces cerevisiae under saline stress[J].Extremophiles,2005,9(1):7-16.
[10] HOTTLGERH T,DE VIRGILIOV C,HALL M N,et al.The role of trehalose synthesis for the acquisition of thermotolerance in yeast:Ⅱ.physiological concentrations of trehalose increase the thermal stability of proteins in vitro[J].Eur J Biochem,1994,219(1/2):187-193.
[11] SIMOLA M,H?NNINEN A L,STRANIUS S M,et al.Trehalose is required for conformational repair of heat-denatured proteins in the yeast endoplasmic reticulum but not for maintenance of membrane traffic functions after severe heat stress[J].Mol Microbiol,2000,37(1):42-53.
[12] XU C,ZHAO X Q,YUAN W J.Improving ethanol tolerance of a self-flocculating yeast optimization of medium composition[J].World J Microbiol Biotechnol,2008,24:2257-2261.
[13] MICHAILLAT L,MAYER A.Identification of genes affecting vacuole membrane fragmentation in Saccharomyces cerevisiae[J].PLoS ONE,2013,8(2):e54160.
[14] PETITJEAN M,TESTE M A,FRANCOIS J M,et al.Yeast tolerance to various stresses relies on the trehalose-6P synthase(Tps1)protein,not on trehalose[J].J Biol Chem,2015,26(10):16177-16190.
[15] 马荣山,王珊珊.酱醪中耐高温耐盐酵母菌分离鉴定[J].食品工业科技,2013,34(14):191-196.
[16] 李艳玲,史仁玖,张显忠,等.蒽酮-硫酸法测定泰山灰树花中海藻糖含量的研究[J].食品科学,2009,30(8):218-220.
[17] 桑宏庆,于天宇,安乐.磷钼蓝分光光度法测定果蔬中有机磷[J].饮料工业,2011,14(8):30-34.
[18] BRADFORD M M.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J].Anal Biochem,1976,72(1/2):248-254.
[19] 李栎,纪春艳,王振中.水稻叶片质膜蛋白的提取与纯化[J].江苏农业科学,2013,41(6):44-46.
[20] 郜希璐.白酒窖泥中酵母菌的分离鉴定及发酵特性研究[D].天津:天津大学,2009.
[21] 陈丽君,肖冬光,郭学武,等.面包酵母海藻糖含量与酵母耐性之间的关系[J].食品工业科技,2011,32(8):112-114.
[22] HERRERA R,SALAZAR A,RAMOS-MORENO L,et al.Vacuolar control of subcellular cation distribution is a key parameter in the adaptation of Debaryomyces hansenii to high salt concentrations[J].Fungal Genet Biol,2017,100:52-60
[23] KERKEB L,DONAIRE J P,RODRíGUE-ROSALES P M.Plasma membrane H+-ATPase activity is involved in adaptation of tomato calli to NaCl[J].Physiol Plant,2001,111(4):483-490.
[24] 杨颖丽,张峰,张立新,等.盐胁迫对小麦根质膜ATPase活性的影响[J].西北植物学报,2003,23(3):401-405.

相似文献/References:

[1]杨成,陈名君,刘辉,等.斜链拟青霉多糖活性组分的分离及其清除自由基活性[J].生物加工过程,2013,11(05):67.[doi:10.3969/j.issn.1672-3678.2013.05.013]
 YANG Cheng,CHEN Mingjun,LIU Hui,et al.Isolating and free radical scavenging active fractions of polysaccharide from Paecilomyces cateniobliquus[J].Chinese Journal of Bioprocess Engineering,2013,11(06):67.[doi:10.3969/j.issn.1672-3678.2013.05.013]
[2]熊强,杨青丽,韩浩.人源双歧杆菌的分离纯化及鉴定[J].生物加工过程,2013,11(06):38.[doi:10.3969/j.issn.1672-3678.2013.06.008]
 XIONG Qiang,YANG Qingli,HAN Hao.The identification of Bifidobacterium form human feces[J].Chinese Journal of Bioprocess Engineering,2013,11(06):38.[doi:10.3969/j.issn.1672-3678.2013.06.008]
[3]付建红,杨新平,谢芹,等.奇台黑碱土可培养细菌的分离及抑制黑色素合成活性菌株的筛选[J].生物加工过程,2017,15(06):74.[doi:10.3969/j.issn.1672-3678.2017.06.011]
 FU Jianhong,YANG Xinping,XIE Qin,et al.Isolation of culturable bacteria from saline-alkali soil in Qitai and screening of strains of inhibiting melanin biosynthesis[J].Chinese Journal of Bioprocess Engineering,2017,15(06):74.[doi:10.3969/j.issn.1672-3678.2017.06.011]

备注/Memo

备注/Memo:
收稿日期:2017-08-27修回日期:2018-01-08
基金项目:国家重点基础研究发展计划(973计划)(2013CB733904); 江苏省自然科学基金(BK20130932)
作者简介:韦元琪(1992—),女,江苏南通人,硕士研究生,研究方向:酵母耐受机制; 蔡恒(联系人),副教授,E-mail:cheng@njtech.edu.cn
引文格式:韦元琪,钱旭,雍晓雨,等.一株耐盐酵母的分离、鉴定及其生物学特性[J].生物加工过程,2018,16(6):1-7.
WEI Yuanqi,QIAN Xu,YONG Xiaoyu,et al.Isolation,identification and characterization of a salt tolerant yeast[J].Chin J Bioprocess Eng,2018,16(6):1-7..
更新日期/Last Update: 2018-11-30