|本期目录/Table of Contents|

[1]孙骏飞,周成,何冰芳,等.菊糖芽孢乳杆菌利用麸皮水解液发酵生产D-乳酸[J].生物加工过程,2017,15(01):1-7.[doi:10.3969/j.issn.1672-3678.2017.01.001]
 SUN Junfei,ZHOU Cheng,HE Bingfang,et al.D-lactic acid fermentation using hydrolysate of wheat bran by Sporolactobacillus inulinus[J].Chinese Journal of Bioprocess Engineering,2017,15(01):1-7.[doi:10.3969/j.issn.1672-3678.2017.01.001]
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菊糖芽孢乳杆菌利用麸皮水解液发酵生产D-乳酸()
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
15
期数:
2017年01期
页码:
1-7
栏目:
出版日期:
2017-01-30

文章信息/Info

Title:
D-lactic acid fermentation using hydrolysate of wheat bran by Sporolactobacillus inulinus
文章编号:
1672-3678(2017)01-0001-07
作者:
孙骏飞1周成2何冰芳3吴斌1
1.南京工业大学 生物与制药工程学院,江苏 南京 211800; 2.扬子江药业集团有限公司,江苏 泰州 225321; 3.南京工业大学 药学院,江苏 南京 211800
Author(s):
SUN Junfei1ZHOU Cheng2HE Bingfang3WU Bin1
1.College of Biotechnology and Pharmaceutical Engineering,Nanjing Tech University,Nanjing 211800,China; 2.Yangtze River Pharmaceutical Group,Taizhou 225321,China; 3.College of Pharmacy,Nanjing Tech University,Nanjing 211800,China
关键词:
D-乳酸 麸皮 菊糖芽孢乳杆菌 碳源 氮源
分类号:
Q815
DOI:
10.3969/j.issn.1672-3678.2017.01.001
文献标志码:
A
摘要:
考察菊糖芽孢乳杆菌YBS1-5利用麸皮的水解液发酵生产D-乳酸的性能。首先研究了不同蛋白酶对麸皮中蛋白组分的水解效率,优选酸性蛋白酶并对其进行水解工艺的优化,最终其水解液中的含氮量为4.6 g/L,水解效率为85.8%。对酸性蛋白酶的水解液残渣进行稀酸预处理后,利用纤维素酶对其进行酶解。通过批次补料酶解,水解液中的还原糖质量浓度达141.2 g/L,其中葡萄糖质量浓度为138.1 g/L、木糖质量浓度为1.4 g/L。利用麸皮的蛋白酶水解液和纤维素酶水解液替代葡萄糖和酵母粉发酵制备D-乳酸。在96 h内,D-乳酸产量达99.5 g/L,生产速率达1.04 g/(L·h),转化率89.1%。

参考文献/References:

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相似文献/References:

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备注/Memo

备注/Memo:
收稿日期:2016-04-19
基金项目:国家重点基础研究发展计划(973计划)(2011CB707405); 江苏省自然科学基金(BK201411456)
作者简介:孙骏飞(1990—),男,河北沧州人,研究方向:生物化工; 吴斌(联系人),副研究员,E-mail:wubin1977@njtech.edu.cn.
更新日期/Last Update: 2017-01-30