|本期目录/Table of Contents|

[1]徐刚,李牧,孙苗苗,等.立体选择性脂肪酶的筛选及其在薄荷醇拆分中的应用[J].生物加工过程,2013,11(01):1-4.[doi:10.3969/j.issn.1672-3678.2013.01.001]
 XU Gang,LI Mu,SUN Miaomiao,et al.Screening of stereoselective lipase and its application in resolution of menthol[J].Chinese Journal of Bioprocess Engineering,2013,11(01):1-4.[doi:10.3969/j.issn.1672-3678.2013.01.001]
点击复制

立体选择性脂肪酶的筛选及其在薄荷醇拆分中的应用()
分享到:

《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
11
期数:
2013年01期
页码:
1-4
栏目:
出版日期:
2008-01-30

文章信息/Info

Title:
Screening of stereoselective lipase and its application in resolution of menthol
文章编号:
1672-3678(2013)01-0001-04
作者:
徐刚李牧孙苗苗吴坚平杨立荣
浙江大学 化学工程与生物工程学系,杭州 310027
Author(s):
XU GangLI MuSUN MiaomiaoWU JianpingYANG Lirong
Department of Chemical and Biological Engineering,Zhejiang University,Hangzhou 310027,China
关键词:
脂肪酶 L-薄荷醇 筛选 产碱假单肥菌 立体选择性
分类号:
Q556+.1
DOI:
10.3969/j.issn.1672-3678.2013.01.001
文献标志码:
A
摘要:
采用双平板透明圈方法,筛选得到1株能够选择性水解L-薄荷醇丙酸酯的微生物。通过16sDNA测序,鉴定为产碱假单胞菌Pseudomonas alcaligenes。 该菌所产脂肪酶可水解4对薄荷醇丙酸酯,在35 ℃时绝对转化率达到50%时,表现出较高的对映体过量值(e.e.p>99%)与非对映体过量值(d.e.p>90%)。这一结果表明,产碱假单胞菌脂肪酶是具有工业应用前景的生物催化剂。

参考文献/References:

[1] Zheng Gaowei,Yu Huilei,Zhang Jiandong,et al.Enzymatic production of l-menthol by a high substrate concentration tolerable esterase from newly isolated Bacillus subtilis ECU0554[J].Adv Syn Catal,2009,351:405-414.
[2] Tani K,Yamagat T,Akutagawa S.Highly enantioselective isomerization of prochiral allylamines catalyzed by chiral diphosphine rhodium(I)complexes:preparation of optically active enamines[J].J Am Chem Soc,1984,106:5208-5217.
[3] Yu Lijuan,Xu Yan.Highly enantioselective hydrolysis of dl-menthyl acetate to l-menthol by whole-cell lipase from Burkholderia cepacia ATCC 25416[J].J Mol Catal B:Enzymatic,2007,47(3):149-154
[4] Vorlova S,Bornscheuer U T,Gatfield I,et al.Enantioselective hydrolysis of D,L-(±)menthyl benzoate to L-(-)-menthol by recombinant Candida rugosa lipase LIP1[J].Adv Syn Catal,2002,344(10):1152-1155.
[5] Gao Renjun,Feng Yan.Cloning,purification and properties of a hyperthermophilic esterase from archaeon Aeropyrum pernix K1[J].J Mol Catal B:Enzymatic,2003,24:1-8.
[6] Bradya D,Reddya S.Biocatalytic enantiomeric resolution of l-menthol from an eight isomeric menthol mixture through transesterification[J].J Mol Catal B:Enzymatic,2012,75:1-10.
[7] Lorenz P,Liebeton K,Niehaus F.Screening for novel enzymes for biocatalytic processes:accessing the metagenome as a resource of novel functional sequence space[J].Curr Opin Biotechnol,2002,13:572-577.
[8] Baharum S N,Salleh A B,Razak C N A.Organic solvent tolerant lipase by Pseudomonas sp.strain S5:stability of enzyme in organic solvent and physical factors affecting its production[J].Ann Microbiol,2003,53:75-83.
[9] Bozoglu F,Swaisgood H E,Adams D M.Isolation and characterization of an extracellular heat stable lipase produced by Pseudomonas fluorescens MC50[J].Agric Food Chem,1984,32:2-6.

相似文献/References:

[1]潘雪,谭天伟.假丝酵母99-125脂肪酶喷雾干燥工艺及催化性质的研究[J].生物加工过程,2010,8(02):30.[doi:10.3969/j.issn.1672-3678.2010.02.006]
 PAN Xue,TAN Tianwei.Spray drying process for formulation of lipase from Candida sp. 99-125 and catalytic properties the powdered lipase[J].Chinese Journal of Bioprocess Engineering,2010,8(01):30.[doi:10.3969/j.issn.1672-3678.2010.02.006]
[2]刘信宁,汤鲁宏.L-抗坏血酸洛芬酯非水相酶促合成的动力学与热力学[J].生物加工过程,2010,8(06):33.[doi:10.3969/j.issn.1672-3678.2010.06.007]
 LIU Xin-ning,TANG Lu-hong.[J].Chinese Journal of Bioprocess Engineering,2010,8(01):33.[doi:10.3969/j.issn.1672-3678.2010.06.007]
[3]李全顺,高贵,李广泉,等.脂肪酶催化的末端官能化聚酯合成研究进展[J].生物加工过程,2009,7(02):1.
 undefinedundefinedundefined LI Quan-shun,GAO Gui,LI Guang-quan,et al.Development of lipase-catalyzed synthesis in terminal-functionalized polyesters[J].Chinese Journal of Bioprocess Engineering,2009,7(01):1.
[4]王蕾,蔡宇杰,廖祥儒,等.Geotrichum sp. SYBC WU-3脂肪酶的双水相萃取和酶学性质[J].生物加工过程,2009,7(02):57.
 WANG Lei,CAI Yu-jie,LIAO Xiang-ru,et al.Aqueous two-phase extraction of lipase produced by Geotrichum sp. SYBC WU-3 and characterization of enzyme[J].Chinese Journal of Bioprocess Engineering,2009,7(01):57.
[5]郭炳其,段学辉,傅奇,等.几种离子液体的微波法合成及其对脂肪酶催化效果的影响[J].生物加工过程,2011,9(03):31.[doi:10.3969/j.issn.1672-3678.2011.03.007]
 GUO Bingqi,DUAN Xuehui,FU Qi,et al.Microwave assisted synthesis of ionic liquids and their influence on lipase catalysis[J].Chinese Journal of Bioprocess Engineering,2011,9(01):31.[doi:10.3969/j.issn.1672-3678.2011.03.007]
[6]李全顺,杨艳,于洋,等.脂肪酶催化选择性开环聚合反应研究进展[J].生物加工过程,2011,9(03):71.[doi:10.3969/j.issn.1672-3678.2011.03.015]
 LI Quanshun,YANG Yan,YU Yang,et al.Progress in lipasecatalyzed selective ringopening polymerization[J].Chinese Journal of Bioprocess Engineering,2011,9(01):71.[doi:10.3969/j.issn.1672-3678.2011.03.015]
[7]于大海,王智,郑良玉,等.酶促拆分丁酸环氧丙酯反应体系的筛选[J].生物加工过程,2004,2(01):40.[doi:10.3969/j.issn.1672-3678.2004.01.010]
[8]逄淑召,王群,王智,等.荧光假单孢菌Pseudomonas fluorescence 5963 产脂肪酶条件的优化[J].生物加工过程,2004,2(01):62.[doi:10.3969/j.issn.1672-3678.2004.01.015]
[9]熊健,季峥,吴坚平,等.有机介质中脂肪酶催化转酯化反应拆分苯乙氰醇的研究[J].生物加工过程,2005,3(03):33.[doi:10.3969/j.issn.1672-3678.2005.03.007]
 XIONG Jian,JI Zheng,WU Jian-ping,et al.Resolution of transesterification of mandelonitrile by lipase in organic media[J].Chinese Journal of Bioprocess Engineering,2005,3(01):33.[doi:10.3969/j.issn.1672-3678.2005.03.007]
[10]徐天闻,贾涛,许建和.非水介质中脂肪酶催化的手性拆分研究进展[J].生物加工过程,2005,3(04):1.[doi:10.3969/j.issn.1672-3678.2005.04.001]
 XU Tian-wen,JIA Tao,XU Jian-he.Advancements in chiral resolution catalyzed by lipase in non-aqueous media[J].Chinese Journal of Bioprocess Engineering,2005,3(01):1.[doi:10.3969/j.issn.1672-3678.2005.04.001]

备注/Memo

备注/Memo:
收稿日期:2012-11-07
基金项目:国家重点基础研究发展计划(973计划)资助(2011CB710800); 国家高技术研究发展计划(863计划)资助(2011AA02A209); 国家自然科学基金重点项目资助(20936022)
作者简介:徐刚(1974—),男,山东德州人,副教授,研究方向:手性催化与转化,E-mail:xugang_1030@zju.edu.cn.
更新日期/Last Update: 2013-01-30