|本期目录/Table of Contents|

[1]冯志彬,李昆,石秀峰,等.混菌发酵生产γ-聚谷氨酸的工艺条件优化[J].生物加工过程,2012,10(05):23-28.[doi:10.3969/j.issn.1672-3678.2012.05.005]
　FENG Zhibin,LI Kun,SHI Xiufeng,et al.Optimization of culture conditions for producing poly(γ-glutamic acid)by mixed fermentation[J].Chinese Journal of Bioprocess Engineering,2012,10(05):23-28.[doi:10.3969/j.issn.1672-3678.2012.05.005]
点击复制

混菌发酵生产γ-聚谷氨酸的工艺条件优化()
分享到：

《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
10
期数:
2012年05期
页码:
23-28
栏目:
出版日期:
2012-09-30

文章信息/Info

Title:
Optimization of culture conditions for producing poly(γ-glutamic acid)by mixed fermentation
文章编号:
1672-3678(2012)05-0023-06
作者:
冯志彬; 李昆; 石秀峰; 程仕伟; 陈国忠
鲁东大学 生命科学学院,烟台 264025
Author(s):
FENG Zhibin; LI Kun; SHI Xiufeng; CHENG Shiwei; CHEN Guozhong
College of Life Science, Ludong University, Yantai 264025, China
关键词:
γ-聚谷氨酸;  谷氨酸棒杆菌;  枯草芽胞杆菌
分类号:
TQ921
DOI:
10.3969/j.issn.1672-3678.2012.05.005
文献标志码:
A
摘要:
采用谷氨酸棒杆菌S9114和枯草芽胞杆菌NTG-4在10 L自控发酵罐上进行混菌发酵,探索混菌发酵生产γ-聚谷氨酸的可行性并进行工艺优化。结果表明:温度、接种量、pH及溶氧对聚谷氨酸发酵有较大影响,发酵前期维持32 ℃,6 h提温至37 ℃变温控制,谷氨酸棒杆菌和枯草芽胞杆菌接种量分别为5%和0.5%,pH 7.0,溶氧20%最有利于γ-聚谷氨酸发酵,在此条件下发酵32 h γ-聚谷氨酸最高产量为38.3 g/L。

参考文献/References:

[1] Shih I L,Van Y T.The production of poly(γ-glutamic acid)from microorganisms and its various applications[J].Bioresour Technol,2001,79:207-225.
[2] Huang Baiqi,Qin Peiyong,Xu Zhiwen.Effects of CaCl2 on viscosity of culture broth,and on activities of enzymes around the 2-oxoglutarate branch,in Bacillus subtilis CGMCC 2108producing[J].Bioresour Technol,2011,102:3595-3598.
[3] Chen X,Chen S W.High yield of poly-γ-glutamic acid from Bacillus solid-state fermentation using swine manure as the basis of a solid substrate[J]. Int J Biol Macromol,2005,96:1872-1879.
[4] Du Guocheng,Yang Ge,Qu Yinbo.Effects of glycerol on the production of poly(γ-glutamic acid)by Bacillus licheniformis[J].Proc Biochem,2005,40:2143-2147.
[5] Xu Hong,Jiang Min,Li Hui.Effcient production of poly(γ-glutamic acid)by newly isolated Bacillus subtilis NX-2[J].Proc Biochem,2005,40:519-523.
[6] Wu Qun,Xu Hong,Ying Hanjie.Kinetic analysis and pH-shift control strategy for poly(γ-glutamic acid)production with Bacillus subtilis CGMCC 0833[J].Biochem Eng J,2010,50:24-28.
[7] Shih I L,Van Y T.The production of poly(γ-glutamic acid)from microorganisms and its various applications[J].Bioresour Technol,2001,79:207-225.
[8] Su Yishan,Li Xia,Liu Qingzhi.Improved poly-γ-glutamic acid production by chromosomal integration of the Vitreoscilla hemoglobin gene(vgb)in Bacillus subtilis[J].Bioresour Technol,2010,101:4733-4736.
[9] Shih I L,Wu P J,Shieh C J.Microbial production of a poly(γ-glutamic acid)derivative by Bacillus subtilis[J].Proc Biochem,2005,40:2827-2832.
[10] 胡纯铿,王国川.混合培养发酵L-苹果酸的研究[J].华侨大学学报:自然科学版,2000(1):76-79.
[11] 武改红,赵希景,徐庆阳,等.混合菌发酵法生产三氮唑核苷的工艺条件优化[J].化工学报,2007,58(6):1535-1540.
[12] 徐志南,石峰,岺沛霖.用枯草芽孢杆菌和谷氨酸棒杆菌混合培养体系制备γ-聚谷氨酸的方法:中国,100999756A[P].2007-07-18.
[13] 梁金丰,徐虹,姚俊,等.γ-聚谷氨酸提取的发酵液预处理及分离纯化工艺[J].食品与发酵工业,2009,35(3):6-11.
[14] 冯志彬,程仕伟,缪静,等.γ-PGA生产菌选育及培养条件研究[J].生物加工过程,2010,8(1):40-44.
[15] 冯志彬,刘进杰.提高L-谷氨酸产量的后期发酵工艺参数研究[J].食品科学,2009,30(4):40-43.

相似文献/References:

[1]韩建福,富敏霞,祝铃钰,等.L-苯丙氨酸制备的研究进展[J].生物加工过程,2019,17(02):117.[doi:10.3969/j.issn.1672-3678.2019.02.001]
　HAN Jianfu,FU Minxia,ZHU Lingyu,et al.Advances in L-phenylalanine preparation[J].Chinese Journal of Bioprocess Engineering,2019,17(05):117.[doi:10.3969/j.issn.1672-3678.2019.02.001]
[2]王薛雪,李莎,任战坤,等.γ-聚谷氨酸的抗氧化及对UV辐射致皮肤细胞光老化的修复作用[J].生物加工过程,2020,18(05):561.[doi:10.3969/j.issn.1672-3678.2020.05.003]
　WANG Xuexue,LI Sha,REN Zhankun,et al.Antioxidation of poly-(γ-glutamic acid)and repair of photoaging fibroblasts induced by UV radiation[J].Chinese Journal of Bioprocess Engineering,2020,18(05):561.[doi:10.3969/j.issn.1672-3678.2020.05.003]

备注/Memo

备注/Memo:
收稿日期:2012-05-02
作者简介:冯志彬(1977—),男,山东烟台人,讲师,研究方向:发酵工程,E-mail:fengzhibin257@163.com.
更新日期/Last Update: 2012-09-30