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[1]陈金燕,刘桂艳,黄申,等.含离子液体体系中固定化细胞转化甘草酸生成单葡萄糖醛酸基甘草次酸[J].生物加工过程,2011,9(05):17-21.[doi:doi:10.3969/j.issn.1672-3678.2011.05.004]
 CHEN Jinyan,LIU Guiyan,HUANG Shen,et al.Biosynthesis of glycyrrhetic acid 3-O-mono-β-D-glucuronide by immobilized cells with ionic liquid[J].Chinese Journal of Bioprocess Engineering,2011,9(05):17-21.[doi:doi:10.3969/j.issn.1672-3678.2011.05.004]
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含离子液体体系中固定化细胞转化甘草酸生成单葡萄糖醛酸基甘草次酸()
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
9
期数:
2011年05期
页码:
17-21
栏目:
出版日期:
2011-09-30

文章信息/Info

Title:
Biosynthesis of glycyrrhetic acid 3-O-mono-β-D-glucuronide by immobilized cells with ionic liquid
文章编号:
1672-3678(2011)05-0017-05
作者:
陈金燕1刘桂艳2黄申2童延斌1李春12
1. 石河子大学 化学化工学院,石河子832003;
2. 北京理工大学 生命学院,北京100081
Author(s):
CHEN Jinyan1LIU Guiyan2HUANG Shen2TONG Yanbin1LI Chun12
1. School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832003, China;
2. School of Life Science, Beijing Institute of Technology, Beijing 100081, China
关键词:
葡萄糖醛酸苷酶甘草酸甘草次酸离子液体固定化细胞
分类号:
Q814.2
DOI:
doi:10.3969/j.issn.1672-3678.2011.05.004
文献标志码:
A
摘要:
 研究疏水性离子液体1-丁基-3-甲基咪唑六氟磷酸盐([BMIM]PF6)与醋酸-醋酸钠缓冲液两相体系中,固定化产紫青霉Penicillium purpurogenum Li-3细胞转化甘草酸(GL)生成单葡萄糖醛酸基甘草次酸(GAMG)的反应,并与缓冲液单相体系作为对照。确定了在[BMIM]PF6/缓冲液两相体系中,最适离子液体加入比例、缓冲液pH、反应温度、底物浓度分别为10%、5.8、35 ℃和6.0 mmol/L,在此条件下反应58 h,甘草酸转化率为87.03%,比缓冲液单相体系提高了15.02%。离子液体循环使用8次后,回收利用率维持在85.28%。主产物GAMG和副产物甘草次酸(GA)在两相体系中得到有效分离,为后续产物分离带来便利。

参考文献/References:

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[8]邹树平,张琦,李春,等.水/有机溶剂体系中产紫青霉全细胞催化合成单葡糖醛酸基甘草次酸[J].化工学报,2009,60 (8):2040-2045.
[9]Habulin M,Knez Z.Optimization of (R,S)-1-phenylethanol kinetic resolution over Candida antarctica lipase B in ionic liquids[J].J Mol Catal B:Enzym,2009,58:24-28.
[10]Lou Wenyong,Wang Wei,Li Ruifeng,et al.Efficient enantioselective reduction of 4′-methoxyacetophenone with immobilized Rhodotorula sp.AS2.2241 cells in a hydrophilic ionic liquid-containing co-solvent system[J].J Biotechnol,2009,143(3):190-197.
[11]He Dongmei,Kaleem I,Qin Siying,et al.Biosynthesis of glycyrrhetic acid 3-O-mono-β-D-glucuronide catalyzed by β-D-glucuronidase with enhanced bond selectivity in ionic liquid/buffer biphasic system[J].Process Biochem,2010,45(12):1916-1922.
[12]Matsui S,Matsumoto H,Sonoda Y,et al.Glycyrrhizin and related compounds down-regulate production of inflammatory chemokines IL-8 and eotaxin 1 in a human lung fibroblast cell line[J].Int Immunopharmacol,2004,4(13):1633-1644.

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备注/Memo

备注/Memo:
收稿日期:2011-05-16
基金项目:国家自然科学基金资助项目(20976014);教育部博士点基金资助项目(20091101110036);北京市自然科学基金资助项目(2112035)
作者简介:陈金燕(1983—),女,内蒙古赤峰人,硕士研究生,研究方向:生物催化与酶工程;李春(联系人),教授,E-mail:lichun@bit.edu.cn
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