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[1]凌辉生,李阳,封琳,等.修饰血红素提高血红蛋白类过氧化物酶活性[J].生物加工过程,2011,9(02):68-71.[doi:doi:10.3969/j.issn.1672-3678.2011.02.014]
 LING Huisheng,LI Yang,FENG Lin,et al.Improving peroxidase-like activity of hemoglobin by recombining?hemoglobin using chemically modified heme[J].Chinese Journal of Bioprocess Engineering,2011,9(02):68-71.[doi:doi:10.3969/j.issn.1672-3678.2011.02.014]
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修饰血红素提高血红蛋白类过氧化物酶活性(/HTML)
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
9
期数:
2011年02期
页码:
68-71
栏目:
出版日期:
2011-03-30

文章信息/Info

Title:
Improving peroxidase-like activity of hemoglobin by recombining?hemoglobin using chemically modified heme
文章编号:
1672-3678(2011)02-0068-04
作者:
凌辉生李阳封琳李任强
暨南大学 生物工程学系,广州 510632
Author(s):
LING Huisheng LI Yang FENG Lin LI Renqiang
Department of Biotechnology,Jinan University,Guangzhou 510632,China
关键词:
化学修饰血红素血红蛋白过氧化物酶酶活力
分类号:
Q554
DOI:
doi:10.3969/j.issn.1672-3678.2011.02.014
文献标志码:
A
摘要:
利用苯酚或对羟基联苯对血红蛋白的血红素辅基进行化学修饰,将修饰后的血红素与脱辅基血红蛋白进行重组得到新的血红蛋白。以光吸收扫描分析修饰血红素和重组血红蛋白,证明新的重组血红蛋白构建成功。酶活力测定表明,修饰血红素得到的重组血红蛋白的类过氧化物酶活性都比天然血红蛋白的酶活力高,用对羟基联苯修饰血红素得到的重组血红蛋白的酶活提高明显,约是天然血红蛋白的1.6倍。

参考文献/References:

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[8]Li Y Z,Townshend A.Comparative study of some synthesized and commercial fluorogenic substrates for horseradish peroxidase and its mimetic enzyme hemin by a flow injection method[J].Anal Chim Acta,1997,340(1/2/3):159-168.
[9]丰九英.两种血红素蛋白的辅因子修饰及其结构与功能关系的研究[D].广州:中山大学,2008.
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备注/Memo

备注/Memo:
收稿日期:2010-09-20
基金项目:暨南大学自然科学基金资助项目(109057;640073)
作者简介:凌辉生(1985—),男,江西贵溪人,硕士研究生,研究方向:蛋白质工程;李任强(联系人),教授,E-mail:trqli@jnu.edu.cn
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