|本期目录/Table of Contents|

[1]刘信宁,汤鲁宏.L-抗坏血酸洛芬酯非水相酶促合成的动力学与热力学[J].生物加工过程,2010,8(06):33-39.[doi:10.3969/j.issn.1672-3678.2010.06.007]
 LIU Xin-ning,TANG Lu-hong.[J].Chinese Journal of Bioprocess Engineering,2010,8(06):33-39.[doi:10.3969/j.issn.1672-3678.2010.06.007]
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L-抗坏血酸洛芬酯非水相酶促合成的动力学与热力学(/HTML)
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
8
期数:
2010年06期
页码:
33-39
栏目:
出版日期:
2010-11-30

文章信息/Info

文章编号:
1672-3678(2010)06-0033-07
作者:
刘信宁汤鲁宏
江南大学医药学院,无锡214122
Author(s):
LIU Xin-ningTANG Lu-hong
School of Medicine and Pharmaceutics,Jiangnan University,Wuxi 214122,China
关键词:
脂肪酶洛芬
分类号:
O643.32
DOI:
10.3969/j.issn.1672-3678.2010.06.007
文献标志码:
A
摘要:
对酶法合成L-抗坏血酸洛芬酯(芬维C酯)的反应动力学与热力学进行研究,确定了最有效的酶促反应环境。合成布洛芬维C酯的最优条件:转速200 r/min,温度65 ℃,加酶量5%(以底物的质量分数计),底物浓度1 mol/L,平衡所需时间66 h,平衡时产物质量分数为19.07%;合成酮洛芬维C酯的最优条件:200 r/min,60 ℃,加酶量7.5%,底物浓度600 mmol/L,平衡时间132 h,产物质量分数为10.63%;合成氟比洛芬维C酯的最优条件:200 r/min,65 ℃,加酶量5%,底物浓度400 mmol/L,平衡时间144 h,产物质量分数为6.76%。对底物进行了比较,得到了各自的动力学与热力学参数。布洛芬米氏常数为0.101 μmol/L,vmax=32.68 μmol/(min·g),热力学平衡常数为0.166;酮洛芬的分别为0.144 μmol/L,12.97 μmol/(min·g),0.091;氟比洛芬的分别为0.185 μmol/L,9.35 μmol/(min·g),0.055。

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备注/Memo

备注/Memo:
收稿日期:2010-06-02
基金项目:江苏省无锡市530资助项目
作者简介:刘信宁(1984—),男,山东烟台人,硕士研究生,研究方向:药物合成与生物催化技术;汤鲁宏(联系人),副教授,Email:tangluhong@msn.comL
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