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[1]姚嘉旻,姜岷,吴昊,等.稀酸水解玉米芯制备丁二酸[J].生物加工过程,2010,8(03):66-72.[doi:10.3969/j.issn.1672-3678.2010.03.014]
 YAO Jia-min,JIANG Min,WU Hao,et al.Preparation of saccharification liquid for succinic acid by fermentation from corncob sulfuric acid hydrolysis[J].Chinese Journal of Bioprocess Engineering,2010,8(03):66-72.[doi:10.3969/j.issn.1672-3678.2010.03.014]
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稀酸水解玉米芯制备丁二酸
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
8
期数:
2010年03期
页码:
66-72
栏目:
出版日期:
2010-05-30

文章信息/Info

Title:
Preparation of saccharification liquid for succinic acid by fermentation from corncob sulfuric acid hydrolysis
文章编号:
1672-3678(2010)03-0066-07
作者:
姚嘉旻姜岷吴昊陈可泉
南京工业大学 生物与制药工程学院 材料化学工程国家重点实验室,南京210009
Author(s):
YAO Jia-minJIANG MinWU HaoCHEN Ke-quan
State Key Laboratory of Materials-Oriented Chemical Engineering,College of Biotechnology and Pharmaeutical Engineering,Nanjing University of echnology,Nanjing 210009,China
关键词:
水解脱毒脱盐玉米芯发酵丁二酸
分类号:
TQ921
DOI:
10.3969/j.issn.1672-3678.2010.03.014
文献标志码:
A
摘要:
利用正交设计得到稀H2SO4水解玉米芯制备混合糖液的优化工艺:玉米芯料液比1∶5(质量体积比),物料粒径250~380 μm、H2SO4用量3%(体积分数)、水解温度126 ℃、反应时间2.5 h。此工艺条件下的总糖收率达90%,总糖质量浓度为60 g/L,发酵抑制物糠醛含量为0.87 g/L,5-羟甲基糠醛含量为0.68 g/L。在此基础上利用活性炭吸附和Ca(OH)2中和对玉米芯混合糖液进行脱毒及脱盐处理,SO2-4脱除率达96%,色素脱除率为96%,糠醛、5-羟甲基糠醛及多酚类物质脱除率均高于50%。处理后的玉米芯多组分糖液作为产琥珀酸放线杆菌(Actinobacillus succinogenes)NJ113的发酵C源,当培养基中初始总糖质量浓度为50 g/L时,丁二酸收率为61.68%,丁二酸质量浓度为30.8 g/L;初始总糖质量浓度为70 g/L时,丁二酸收率仍可达50%以上,丁二酸质量浓度为35.2 g/L。发酵实验表明,将经过脱毒脱盐处理的玉米芯多组分糖液替代葡萄糖作为C源发酵制备丁二酸具有可行性。

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2009-02-24
基金项目:国家高技术研究发展计划(863计划)资助项目(2006AA02Z235);国家自然科学基金资助项目(20606017);国家自然科学基金重点资助项目(20336010);江苏省高校自然科学研究计划资助项目(05KJB180043)
作者简介:姚嘉旻(1981—),男,江苏淮安人,硕士研究生,研究方向:生物发酵工艺;姜岷(联系人),教授,E-mail:jiangmin@njut.edu.cn
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