|本期目录/Table of Contents|

[1]黄仁术,倪菊芳.胆红素提取工艺中的皂化水解条件[J].生物加工过程,2010,8(03):18-22.[doi:10.3969/j.issn.1672-3678.2010.03.004]
 HUANG Ren-shu,NI Ju-fang.Basic hydrolysis of bilirubin in extraction process[J].Chinese Journal of Bioprocess Engineering,2010,8(03):18-22.[doi:10.3969/j.issn.1672-3678.2010.03.004]
点击复制

胆红素提取工艺中的皂化水解条件
(/HTML)
分享到:

《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
8
期数:
2010年03期
页码:
18-22
栏目:
出版日期:
2010-05-30

文章信息/Info

Title:
Basic hydrolysis of bilirubin in extraction process
文章编号:
1672-3678(2010)03-0018-05
作者:
黄仁术倪菊芳
皖西学院 化学与生命科学系,六安 237012
Author(s):
HUANG Ren-shuNI Ju-fang
Department of Chemistry and Life Science, West Anhui University, Liu′an 237012, China

关键词:
胆红素水解提取工艺
分类号:
R284.2
DOI:
10.3969/j.issn.1672-3678.2010.03.004
文献标志码:
A
摘要:
为提高胆红素生产效益,采取正交实验法,优化皂化水解pH、温度和时间。结果表明:在pH 12.0、105 ℃、20 min时,胆红素粗制品收率平均为0.866 7 g/L,但纯度仅为24.01%,折合纯品收率为0.208 1 g/L。胆红素的纯度在pH 11.0、100 ℃、10~30 min时,平均纯度为67.91%,但粗制品收率仅为0.402 2 g/L,折合纯品收率为0.273 1 g/L。胆红素的折合纯品收率,在pH 10~12、100 ℃、20 min时,平均可达0.366 8 g/L,粗制品收率为0.568 9 g/L、纯度64.12%,此时胆红素的生产效益比传统方法提高了43.9%。

参考文献/References:

[1]童晓滨,陈良壁.提取胆红素工艺方法的改进及其机理探讨[J].天水师范学院学报,2006,26(2):54-56.
Tong Xiaobin,Chen Liangbi.The improvement of technological method in extracting bilirubin and the study of its mechanisms[J].Journal of Tianshui Normal University,2006,26(2):54-56.
[2]林桂金.醋酸法提取胆红素的工艺研究[J].中国医药导报,2007,4(27):104-105.
Lin Guijin.Process studies on extraction of bilirubin by acetic acid[J].China Medical Bulletin,2007,4(27):104-105.
[3]夏小庆,王素华,方骏.胆汁中提取胆红素的最佳实验条件探讨[J].皖南医学院学报,1996,15(3):201-203.
Xia Xiaoqing,Wang Suhua,Fang Jun.The selection of the best conditions for recovering bilirubin from the bile[J].Journal of Wannan Medical College,1996,15(3):201-203.
[4]刘荣华,汪洋,陈兰英.CDA-40大孔树脂提取胆红素工艺研究[J].中成药,2000,22(3):187-190.
Liu Ronghua,Wang Yang,Chen Lanying.Study on extraction process of bilirubin with CDA-40 macroporous resin[J].Chinese Traditional Patent Medicine,2000,22(3):187-190.
[5]刘东明.猪活体胆汁引流及胆红素生产工艺的研究[D].哈尔滨:东北农业大学,2001.
[6]王少圣,徐宜萍.胆红素提取方法探讨[J].基层中药杂志,1999(1):35-36.
Wang Shaosheng,Xu Yiping.Explore the method of extracting bilirubin[J].Primary Journal of Chinese Materia Medica,1999(1):35-36.
[7]张丽萍,李良玉,杨楠,等.超声波辅助提取猪胆红素技术条件的研究[J].食品与药品,2009,11(7):29-32.
Zhang Liping, Li Liangyu, Yang Nan, et al. Study on extraction technology for bilirubin by ultrasonic assist[J]. Food and Drug, 2009,11(7):29-32.
[8]韩伟,庄桂东,仇宏伟,等.超临界CO2和乙酸乙酯提取胆红素[J].精细化工,2009,26(11):1072-1076.
Han Wei, Zhuang Guidong, Qiu Hongwei, et al. Study on the extraction of bilirubin by supercritical CO2 extraction and ethyi acetate[J]. Fine Chemicals, 2009,26(11):1072-1076.
[9]孟兆新,梁晓舟,刘慧,等.胆红素的生物制取[J].辽宁医药,2004(4):25-27.
Meng Zhaoxin,Liang Xiaozhou,Liu Hui,et al.Study on the bioleaching of bilirubin[J].Liaoning Medicine,2004(4):25-27.

相似文献/References:

[1]宋娜娜,宋向阳,欧阳嘉,等.里氏木霉与黑曲霉混合发酵产纤维素酶及其水解特性[J].生物加工过程,2010,8(05):5.[doi:doi:10.3969/j.issn.1672-3678.2010.05.002]
 SONG Na-na,SONG Xiang-yang,OUYANG Jia,et al.Cellulose production from fungal mixed culture of Trichoderma reesei RUT C-30 and Apsergillus niger NL02 on steam-exploded corn stover and characteristics of hydrolysis[J].Chinese Journal of Bioprocess Engineering,2010,8(03):5.[doi:doi:10.3969/j.issn.1672-3678.2010.05.002]
[2]巩桂芬,张明玉,邢立新,等.不同纤维素原料超临界水解的研究[J].生物加工过程,2010,8(02):8.[doi:1672- 3678( 2010) 02- 0008- 05]
 GONG Guifen,ZHANG M ingyu,et al.Hydrolysis of lignocellulosic feedstock with supercriticalwater[J].Chinese Journal of Bioprocess Engineering,2010,8(03):8.[doi:1672- 3678( 2010) 02- 0008- 05]
[3]王卿卿,冯惠勇,康辉,等.耐高温β-糖苷酶的乳糖水解活性及转糖基活性[J].生物加工过程,2009,7(01):49.
 WANG Qing-qing,FENG Hui-yong,KANG Hui,et al.Activities of thermostable β-glucosidase in lactose hydrolysis and transgalactosylation[J].Chinese Journal of Bioprocess Engineering,2009,7(03):49.
[4]姚嘉旻,姜岷,吴昊,等.稀酸水解玉米芯制备丁二酸[J].生物加工过程,2010,8(03):66.[doi:10.3969/j.issn.1672-3678.2010.03.014]
 YAO Jia-min,JIANG Min,WU Hao,et al.Preparation of saccharification liquid for succinic acid by fermentation from corncob sulfuric acid hydrolysis[J].Chinese Journal of Bioprocess Engineering,2010,8(03):66.[doi:10.3969/j.issn.1672-3678.2010.03.014]
[5]吕浩,邢楠楠,林增祥,等.固定化纤维素酶对预处理纤维素原料水解效果的影响[J].生物加工过程,2011,9(05):38.[doi:doi:10.3969/j.issn.1672-3678.2011.05.008]
 LU Hao,XING Nannan,LIN Zengxiang,et al.Effect of immobilized cellulase on evaluating hydrolysis efficiency of pretreated cellulose material[J].Chinese Journal of Bioprocess Engineering,2011,9(03):38.[doi:doi:10.3969/j.issn.1672-3678.2011.05.008]
[6]李星纬,巩桂芬,李晓东. 超临界下有机酸对稻秆水解糖化的影响[J].生物加工过程,2012,10(02):11.[doi:10.3969/j.issn.1672-3678.2012.02.003]
 LI Xingwei,GONG Guifen,LI Xiaodong. Effects of organic acids on rice straw on supercritical hydrolysis[J].Chinese Journal of Bioprocess Engineering,2012,10(03):11.[doi:10.3969/j.issn.1672-3678.2012.02.003]
[7]朱跃钊,卢定强,万红贵,等.木质纤维素预处理技术研究进展[J].生物加工过程,2004,2(04):11.[doi:10.3969/j.issn.1672-3678.2004.04.003]
[8]吴永宏,肖明芳,吕浩,等.核酸酶P1催化水解热变性DNA的研究[J].生物加工过程,2005,3(02):53.[doi:10.3969/j.issn.1672-3678.2005.02.012]
 WU Yong-Hong,XIAO Ming-fang,LU Hao,et al.Studies on enzymatic hydrolysis of the heat-denatured DNA by nuclease P1[J].Chinese Journal of Bioprocess Engineering,2005,3(03):53.[doi:10.3969/j.issn.1672-3678.2005.02.012]
[9]XU Juan,袁艳娟,TU Chun-yan.胃蛋白酶水解绿豆分离蛋白的工艺[J].生物加工过程,2008,6(04):31.[doi:10.3969/j.issn.1672-3678.2008.04.006]
 XU Juan,YUAN Yan-juan,TU Chun-yan.Preparation of oligo-peptide by pepsin hydrolyzation of proteins isolated from mung bean[J].Chinese Journal of Bioprocess Engineering,2008,6(03):31.[doi:10.3969/j.issn.1672-3678.2008.04.006]
[10]朱耀光,谭天伟.豆粕水解液为氮源发酵产脂肪酶的研究[J].生物加工过程,2008,6(05):36.[doi:10.3969/j.issn.1672-3678.2008.05.008]
 ZHU Yao-guang,TAN Tian-wei.Lipase production by using hydrolyzed soybean meal as nitrogen source[J].Chinese Journal of Bioprocess Engineering,2008,6(03):36.[doi:10.3969/j.issn.1672-3678.2008.05.008]

备注/Memo

备注/Memo:
收稿日期:2009-10-14
基金项目:安徽省高等学校省级自然科学研究项目(KJ2009B087)
作者简介:黄仁术(1976—),女,新疆阿克苏人,硕士,讲师,研究方向:生物资源开发,E-mail:ahhrs@126.com
更新日期/Last Update: