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[1]徐国英,林学政,王能飞,等.产低温蛋白酶极地菌株的筛选及Pseudoalteromonas sp. QI-1产蛋白酶粗酶性质[J].生物加工过程,2010,8(02):55-60.[doi:10.3969/j.issn.1672-3678.2010.02.011]
　XU Guoying,LIN Xuezheng,WANG Nengfei,et al.Screening of cold active protease strains from polar microorganism and partialcharacteristics of crude protease excreted by Pseudoalteromonas sp. QI-1[J].Chinese Journal of Bioprocess Engineering,2010,8(02):55-60.[doi:10.3969/j.issn.1672-3678.2010.02.011]
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产低温蛋白酶极地菌株的筛选及Pseudoalteromonas sp. QI-1产蛋白酶粗酶性质(/HTML)
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
8
期数:
2010年02期
页码:
55-60
栏目:
出版日期:
2010-03-30

文章信息/Info

Title:
Screening of cold active protease strains from polar microorganism and partial
characteristics of crude protease excreted by Pseudoalteromonas sp. QI-1
作者:
徐国英;林学政;王能飞;朱启忠
1. 山东大学威海分校海洋学院，威海264209；2. 国家海洋局第一海洋研究所海洋生物活性物质重点实验室，青岛266061
Author(s):
XU Guoying1; 2;  LIN Xuezheng2;  WANG Nengfei2;  ZHU Qizhong1
1.  Marine College,Shandong University at Weihai, Weihai 264209, China;2. Key Laboratory of Marine Bioactive Substances,First Institute of Oceanography, SOA, Qingdao 266061, China
关键词:
低温蛋白酶; 酶学性质; 系统发育分析; 极地微生物
分类号:
Q94
DOI:
10.3969/j.issn.1672-3678.2010.02.011
文献标志码:
A
摘要:
通过酪蛋白平板法从实验室极地微生物资源库中筛选到130株在低温条件(4℃)下具有蛋白酶活性的菌株,并对部分酪蛋白水解圈较大的菌株进行了酶活测定和系统发育分析。发现酶活较高的8株菌分别属于假交替单胞菌属(Pseudoalteromonas)、科尔韦尔氏菌属(Colwellia)、希瓦氏菌属(Shewanella)、嗜冷杆菌属(Psychrobacter)。选择低温蛋白酶活性较高的菌株Pseudoalteromonas sp.QI-1为研究对象,以酪蛋白为反应底物对其所产低温蛋白酶粗酶酶性质进行初步研究。结果表明:QI-1低温蛋白酶酶活最适反应温度为40℃,在0℃时保持10%的相对酶活,酶活最适反应pH为10.0;其催化作用不需要金属离子的参与;热稳定性极差,在60℃放置15 min即完全失活。

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