|本期目录/Table of Contents|

[1]潘雪,谭天伟.假丝酵母99-125脂肪酶喷雾干燥工艺及催化性质的研究[J].生物加工过程,2010,8(02):30-35.[doi:10.3969/j.issn.1672-3678.2010.02.006]
 PAN Xue,TAN Tianwei.Spray drying process for formulation of lipase from Candida sp. 99-125 and catalytic properties the powdered lipase[J].Chinese Journal of Bioprocess Engineering,2010,8(02):30-35.[doi:10.3969/j.issn.1672-3678.2010.02.006]
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假丝酵母99-125脂肪酶喷雾干燥工艺及催化性质的研究(/HTML)
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
8
期数:
2010年02期
页码:
30-35
栏目:
出版日期:
2010-03-30

文章信息/Info

Title:
Spray drying process for formulation of lipase from Candida sp. 99-125 and catalytic properties the powdered lipase
作者:
潘雪;谭天伟
北京化工大学生命科学与技术学院北京市生物加工过程重点实验室,北京100029
Author(s):
PAN Xue TAN Tianwei
Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical
Technology, Beijing 100029, China
关键词:
假丝酵母脂肪酶响应面法喷雾干燥
分类号:
Q556.1 TQ922.1
DOI:
10.3969/j.issn.1672-3678.2010.02.006
文献标志码:
A
摘要:
利用响应面法对假丝酵母脂肪酶喷雾干燥工艺条件进行优化,考察进口温度、雾化速度、保护剂含量对脂肪酶活力收率的影响。确定了最佳喷雾条件:保护剂为10~15 g/L的阿拉伯胶,进口温度115~120℃,雾化速度0.4 L/h,可得到收率最高为60.5%的脂肪酶酶粉。制得的固定化酶用于手性拆分(R,S)-1-苯乙醇,光学产率最高可达到53.6%;用于催化合成生物柴油,转化率最高可达到90.2%。在4、30℃下密封保存,半衰期可分别达到15个月、3个月。

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