|本期目录/Table of Contents|

[1]曹丹,王学东,张鲁嘉,等.产唾液酸酶微生物的筛选及产酶条件的优化[J].生物加工过程,2009,7(04):40-45.
 CAO Dan,WANG Xue-dong,ZHANG Lu-jia,et al.Screening of sialidase-producing microorganism and optimization of sialidase production[J].Chinese Journal of Bioprocess Engineering,2009,7(04):40-45.
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产唾液酸酶微生物的筛选及产酶条件的优化
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
7
期数:
2009年04期
页码:
40-45
栏目:
出版日期:
2009-07-30

文章信息/Info

Title:
Screening of sialidase-producing microorganism and optimization of sialidase production
文章编号:
1672-3678(2009)04-0040-06
作者:
曹丹王学东张鲁嘉张建国魏东芝
华东理工大学 生物反应器工程国家重点实验室,上海 200237
Author(s):
CAO Dan WANG Xue-dong ZHANG Lu-jia ZHANG Jian-guo WEI Dong-zhi
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
关键词:
唾液酸酶生物转化单唾液酸四己糖神经节苷脂溶黄嘌呤厄菌
分类号:
Q815; Q819
文献标志码:
A
摘要:
以无单唾液酸四己糖神经节苷脂(GM1)的神经节苷脂为唯一C源,从土壤中筛选出1株能以多唾液酸神经节苷脂为底物,转化生成GM1的产唾液酸酶微生物,经鉴定为溶黄嘌呤厄菌(Oerskovia xanthineolytica)。利用单因素法和响应面分析法对溶黄嘌呤厄菌产生唾液酸酶的条件进行优化,酶活提高了4.89倍。利用优化后培养条件,以神经节苷脂混合体为底物,经过微生物生物转化后,单唾液酸神经节苷脂GM1的含量从10%提高到83.7%。

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2008-11-06
基金项目:国家高技术研究发展计划(863计划)重点资助项目(2009ZX09306-001)
作者简介:曹丹(1983—),女,浙江上虞人,硕士研究生,研究方向:应用微生物;王学东(联系人),副研究员,E-mail: xdwang@ecust.edu.cn
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