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[1]任梦远,白姝,孙彦.离子液体中固定化脂肪酶催化拆分(±)-薄荷醇[J].生物加工过程,2009,7(03):16-20.
 REN Meng-yuan,BAI Shu,SUN Yan.Resolution of (±)-menthol in ionic liquid catalyzed by immobilized lipase on magnetic microspheres[J].Chinese Journal of Bioprocess Engineering,2009,7(03):16-20.
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离子液体中固定化脂肪酶催化拆分(±)-薄荷醇
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
7
期数:
2009年03期
页码:
16-20
栏目:
出版日期:
2009-05-30

文章信息/Info

Title:
Resolution of (±)-menthol in ionic liquid catalyzed by immobilized lipase on magnetic microspheres
文章编号:
1672-3678(2009)03-0016-05
作者:
任梦远白姝孙彦
天津大学 化工学院,天津300072
Author(s):
REN Meng-yuan BAI Shu SUN Yan
School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China
关键词:
磁性高分子微球固定化脂肪酶离子液体手性拆分薄荷醇
分类号:
Q814.2
文献标志码:
A
摘要:
以自制的平均粒径为4.5 μm磁性高分子微球为载体,采用离子交换法固定化Candida rugosa脂肪酶,催化(±)-薄荷醇的酯化反应,以考察反应时间、pH、反应温度、水活度等因素对酶的固定化以及酯化反应的影响。在固定化反应150 min、pH 5.0、酯化反应温度30 ℃、固定化酶的水活度为0.78的条件下,所制备的固定化脂肪酶在离子液体[bmim]PF6中催化拆分(±)-薄荷醇的效果最佳,与游离酶相比固定化脂肪酶的立体选择性有很大的提高,对映体过量率可达93%,对映体选择值为35。

参考文献/References:

[1]Park S, Kazlauskas R J. Biocatalysis in ionic liquids-advantages beyond green technology[J]. Current Opinion in Biotechnology, 2003, 14 (4): 432-437.
[2]Lozano P, Piamtongkam R, Kohns K, et al. Ionic liquids improve citronellyl ester synthesis catalyzed by immobilized Candida antarctica lipase B in solvent-free media[J]. Green Chem, 2007, 9(7): 780-784.
[3]Liu B K, Wu Q, Xu J M, et al. N-Methylimidazole significantly improves lipase-catalysed acylation of ribavirin[J]. Chem Commun, 2007: 295-297.
[4]Kim M J, Choi M Y. Enzymatic selective acylation of glycosides in ionic liquids: significantly enhanced reactivity and regioselectivity[J]. J Mol Catal B: Enzyme, 2003, 26: 115-118.
[5]Yuan Y, Bai S, Sun Y. Comparison of lipase-catalyzed enantioselective esterification of (±)-menthol in ionic liquids and organic solvents[J]. Food Chemistry, 2006, 97(2): 324-330.
[6]Lu M, Bai S, Yang K, et al. Synthesis and characterization of agnetic polymer microspheres with a core shell structure[J]. China Particuology, 2007, 5(1/2): 180-185.
[7]Huddleston J G, Willauer H D, Swatloki R P. Room temperature ionic liquids as novel media for ‘clean’ liquid-liquid extraction[J]. J Chem Soc Chem Comm, 1998,16: 1765-1766.
[8]Bradford M M. A rapid and sensitive method for the quantition of microgram quantities of protein utilizing the principle of protein-dye binding[J]. Anal Biochem, 1976, 72: 248-254.
[9]Pawinee K, Suree P. Simple assay method for lipase activity and analysis of its catalytic hydrolysis product in water-poor media[J]. Indian J Chem, 1993, 32B: 88-89.
[10]辛嘉英,徐毅,胡霄雪,等. Candida rugosa 脂肪酶同工酶的选择固定化[J]. 离子交换与吸附, 2002, 18(1): 1722.
Xin Jiaying, Xu Yi, Hu Xiaoxue, et al. Selective immobilization of Candida rugosa lipase isoenzyme[J]. Ion Exchange and Adsorption,2002, 18(1): 17-22.
[11]Pronk W, Boswinkel G, Riet K van′t. Parameters influencing hydrolysis kinetics of lipase in a hydrophilic membrane bioreactor[J]. Enzyme Microb Technol, 1992, 14(3): 214-220.
[12]李志国,欧伶,宋庆训,等. 固定化脂肪酶的动力学研究[J]. 华东理工大学学报, 2004, 30(1): 107-110.
Li Zhiguo, Ou Ling, Song Qingxun, et al. Study on the kinetics of immobilized lipase[J]. Journal of East China University of Science and Technology,2004, 30(1): 107-110.
[13]Zhang D H, Bai S, Ren M Y, et al. Optimization of lipase-catalyzed enantioselective esterification of (±)-menthol in ionic liquid[J]. Food Chemistry, 2008, 109(1): 72-80.

相似文献/References:

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 MENG Yan,WU Jianping,XU Gang,et al.Production of L-menthol by immobilized enzyme catalyzeddiastereoselective transesterification[J].Chinese Journal of Bioprocess Engineering,2010,8(03):39.[doi:1672-3678(2010)04-0039-06]
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备注/Memo

备注/Memo:
收稿日期:2008-05-04
基金项目:国家自然科学基金资助项目(20776105)
作者简介:任梦远(1980—),女,山西太原人,硕士研究生,研究方向:酶工程;白姝(联系人),副教授,E-mail:sbai@tju.edu.cn
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