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[1]江波,张江红,李志刚,等.2,3-丁二醇的发酵及盐析分离工艺[J].生物加工过程,2009,7(01):44-48.
 JIANG Bo,ZHANG Jiang-hong,LI Zhi-gang,et al.Fermentation and salting-out Separation of 2,3-butanediol [J].Chinese Journal of Bioprocess Engineering,2009,7(01):44-48.
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2,3-丁二醇的发酵及盐析分离工艺
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
7
期数:
2009年01期
页码:
44-48
栏目:
出版日期:
2009-01-30

文章信息/Info

Title:
Fermentation and salting-out Separation of 2,3-butanediol

文章编号:
1672-3678(2009)01-0044-05
作者:
江波张江红李志刚戴建英修志龙
大连理工大学 环境与生命学院生物科学与工程系,大连116024
Author(s):
JIANG Bo ZHANG Jiang-hong LI Zhi-gang DAI Jian-ying XIU Zhi-long
Department of Bioscience and Biotechnology, School of Environmental and Biological Science and Technology, Dalian University of Technology, Dalian 116024, China
关键词:
23-丁二醇发酵克雷伯氏菌盐析
分类号:
Q815
文献标志码:
A
摘要:
采用克雷伯氏菌(Klebsiella pneumoniae CICC 10011)发酵生产2,3-丁二醇,并对2,3-丁二醇的盐析分离工艺进行了考察。通过实验确定了以葡萄糖为底物微氧批式流加发酵的条件,发酵液中2,3-丁二醇和3-羟基丁酮的质量浓度分别为90.98 g/L和12.40 g/L,2,3-丁二醇的摩尔转化率为82.7%,生产强度达到2.1 g/(L·h)。对发酵液中2,3-丁二醇的盐析分离研究表明,K2HPO4和K3PO4对2,3-丁二醇的盐析效果优于K2CO3。当发酵液浓缩70%后,加入质量分数为45%的K2HPO4,2,3-丁二醇的分配系数达到9~10,回收率为79.37%;上相中2,3-丁二醇的质量浓度达到420 g/L;此时3-羟基丁酮的分配系数和回收率分别为11.9和83.48%。


参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2008-07-07
基金项目:教育部新世纪优秀人才基金资助项目
作者简介:江波(1985—),男,河北邢台人,硕士研究生,研究方向:生物化工;修志龙(联系人),教授,博士生导师,E-mail:zhlxiu@dlet.edu.cn
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