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[1]杜军,纪晓俊,黄和,等.产酸克雷伯氏杆菌发酵产2,3-丁二醇的培养基优化[J].生物加工过程,2009,7(01):34-38.
 DU Jun,JI Xiao-jun,HUANG He,et al.Optimization of fermentation medium for production of 2,3-butanediol by Klebsiella oxytoca[J].Chinese Journal of Bioprocess Engineering,2009,7(01):34-38.
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产酸克雷伯氏杆菌发酵产2,3-丁二醇的培养基优化
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
7
期数:
2009年01期
页码:
34-38
栏目:
出版日期:
2009-01-30

文章信息/Info

Title:
Optimization of fermentation medium for production of 2,3-butanediol by Klebsiella oxytoca
文章编号:
1672-3678(2009)01-0034-05
作者:
杜军纪晓俊黄和胡南任潇聂志奎李霜
南京工业大学制药与生命科学学院,材料化学工程国家重点实验室,南京 210009
Author(s):
DU Jun JI Xiao-jun HUANG He HU Nan REN XiaoNIE Zhi-kui LI Shuang
College of Life Science and Pharmaceutical Engineering, State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing University of Technology, Nanjing 210009, China
关键词:
23-丁二醇产酸克雷伯氏杆菌Plackett-Burman设计响应面法
分类号:
TQ923
文献标志码:
A
摘要:
采用不同设计方法相结合的策略对耐高糖产酸克雷伯氏杆菌(Klebsiella oxytoca) ME-UD-3-4发酵产2,3-丁二醇的培养基进行优化。首先在单因素实验的基础上采用Plackett-Burman设计法对影响ME-UD-3-4发酵产2,3-丁二醇的相关因素进行研究,筛选到3种有显著效应的因素(P<0.05):葡萄糖、玉米浆和MgSO4·7H2O。然后利用响应曲面法(Response Surface Methodology, RSM)对这3种因素的最佳水平范围进一步探讨;对得到的回归模型进行分析,得最佳条件(g/L):葡萄糖220、玉米浆19和MgSO4·7H2O 0.4;在最佳条件下,发酵80 h,2,3-丁二醇产量从原来的57.3 g/L提高到86.1 g/L,生产强度由0.72 g/(L·h)提高到1.08 g/(L·h)。

参考文献/References:

[1]纪晓俊, 朱建国, 高振,等. 微生物发酵法生产 2,3-丁二醇的研究进展[J]. 现代化工, 2006, 26(8):23-27.
Ji Xiaojun,Zhu Jianguo, Gao Zhen, et al. Research progress in the production of 2, 3butanediol by fermentation[J]. Modern Chemical Industry, 2006, 26(8):23-27.
[2]Syu M J. Biological production of 2,3-butanediol[J]. Applied Microbial Biotechnology, 2001, 55(1):10-18.
[3]李元芳, 徐勤, 曹学,等.黏质沙雷氏菌产 2,3-丁二醇培养基的优化[J]. 工业微生物, 2007, 37(3):24-29.
Li Yuanfang,Xu Qin,Cao Xue, et al. Optimization of fermentation medium for 2,3butanediol production by Serratia marcescens[J]. Industrial Microbiology,2007,37(3):24-29.
[4]Ji X J, Huang H, Li S, et al. Enhanced 2,3butanediol production by altering the mixed acid fermentation pathway in Klebsiella oxytoca[J]. Biotechnology Letters, 2008, 30(4):731-734.
[5]杜军, 纪晓俊, 胡南,等. 耐高糖高产 2,3-丁二醇产酸克雷伯氏杆菌的选育[J]. 中国生物工程杂志, 2008,28(6):89-92.
Du Jun, Ji Xiaojun, Hu Nan, et al. Breeding of high glucose tolerant Klebsiella oxytoca for the fermentative production of 2,3-butanediol[J].China Biotechnology, 2008,28(6):89-92.
[6]Weuster D. Experimental design for fermentation media development:statistical design or global random search[J]. J Bioscience and Bioengineering, 2000, 90(5):473-483.
[7]Plackett R L, Burman J P. The design of optimum multifactorial experiments[J]. Biometrika, 1946, 33(4):305-325.
[8]陈魁. 试验设计与分析[M]. 北京:清华大学出版社, 1996:94-180.
[9]胡永红, 沈树宝, 欧阳平凯. 响应面分析法用于微生物培养基浓度的优化[J]. 工业微生物, 2002, 32(1):9-12.
Hu Yonghong, Shen Shubao, Ouyang Pingkai. Application of response surface method for optimal concentration of culture medium for microorganisms[J]. Industrial Microbiology, 2002, 32(1):9-12.

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备注/Memo

备注/Memo:
收稿日期:2008-04-24
基金项目:国家自然科学基金资助项目(20606018);国家重点基础研究发展计划(973计划)资助项目(2007CB707805);国家高技术研究发展计划(863计划)资助项目(2006AA02Z244);江苏省博士后科研资助计划项目(0701035B)
作者简介:杜军(1983— ),男,湖北丹江口人,硕士研究生,研究方向:发酵工程;黄和(联系人),教授,E-mail:biotech@njut.edu.cn
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