|本期目录/Table of Contents|

[1]王宝峰,郑裕国,沈寅初.产S-酰胺酶培养基统计学筛选与响应面优化[J].生物加工过程,2008,6(02):48-52.[doi:10.3969/j.issn.1672-3678.2008.02.009]
 WANG Bao-feng,ZHENG Yu-guo,SHEN Yin-chu.Optimization of medium components for S-amidase production using response surface methodology[J].Chinese Journal of Bioprocess Engineering,2008,6(02):48-52.[doi:10.3969/j.issn.1672-3678.2008.02.009]
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产S-酰胺酶培养基统计学筛选与响应面优化()
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
6
期数:
2008年02期
页码:
48-52
栏目:
出版日期:
2008-03-30

文章信息/Info

Title:
Optimization of medium components for S-amidase production using response surface methodology
作者:
王宝峰郑裕国沈寅初
浙江工业大学, 生物工程研究所, 杭州, 310014
Author(s):
WANG Bao-feng ZHENG Yu-guo SHEN Yin-chu
关键词:
22-二甲基环丙甲酰胺Plackett-Burman设计旋转中心组和设计(CCRD)响应面法(RSM)
分类号:
Q939
DOI:
10.3969/j.issn.1672-3678.2008.02.009
摘要:
利用Design Expert软件中的两水平实验设计和响应面法,对发酵生产S-酰胺酶(可用于拆分2,2-二甲基环丙甲酰胺外消旋体)的培养基进行了优化.采用Plackett-Burman(PB)设计对培养基中相关影响因素的效应进行评价并筛选出了有显著效应的葡萄糖、酵母粉及2, 2-二甲基环丙甲酰胺浓度,其他因素对酰胺酶产量的影响不显著.然后用旋转中心组和实验设计及响应面分析确定了主要影响因素的最佳条件, 在优化的培养基中,酰胺酶产量达到168 U/L, 比优化前的80 U/L提高了110.0%.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:国家重点基础研究发展规划(973计划),国家高技术研究发展计划(863计划)
更新日期/Last Update: 1900-01-01