|本期目录/Table of Contents|

[1]刘桦,张涛,王玉明.金龟子绿僵菌固态发酵产壳聚糖酶[J].生物加工过程,2007,5(03):38-42.[doi:10.3969/j.issn.1672-3678.2007.03.008]
　LIU Hua,ZHANG Tao,WANG Yu-ming.Production of chitosanase by Metarhizium anisopliae in solid state fermentation[J].Chinese Journal of Bioprocess Engineering,2007,5(03):38-42.[doi:10.3969/j.issn.1672-3678.2007.03.008]
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金龟子绿僵菌固态发酵产壳聚糖酶()
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
5
期数:
2007年03期
页码:
38-42
栏目:
出版日期:
2007-08-30

文章信息/Info

Title:
Production of chitosanase by Metarhizium anisopliae in solid state fermentation
作者:
刘桦; 张涛; 王玉明
成都医学院, 医学检验系生物化学与分子生物学教研室, 成都, 610083
Author(s):
LIU Hua;  ZHANG Tao;  WANG Yu-ming
关键词:
金龟子绿僵菌; 固态发酵; 壳聚糖酶; 酶学性质
分类号:
TQ929+.2
DOI:
10.3969/j.issn.1672-3678.2007.03.008
摘要:
对金龟子绿僵菌(Metarhizium anisopliae)AS3.4606产壳聚糖酶进行了固态发酵条件优化及酶学部分特征的研究.正交实验结果表明,以麸皮为碳源,日本根霉菌丝体粉末为氮源,在起始pH 5.0,m(碳):m(氨)为1∶4,m(干重):m(液体)为1:1.4,27 ℃下培养120 h,壳聚糖酶活性可达35.08 U/g(干培养基).粗酶液的最适反应温度为40 ℃,最适反应pH为5.0,酶在pH 5.0～6.0条件下稳定性最高.该酶不能水解固体甲壳素和纤维素粉末,最适底物为胶体壳聚糖.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目：四川省教育厅资助项目
更新日期/Last Update: 1900-01-01