|本期目录/Table of Contents|

[1]张天智,杨炯,杨顺楷,等.新月弯孢霉AS 3.4381对新型甾体底物C11β-羟基化[J].生物加工过程,2006,4(03):22-27.[doi:10.3969/j.issn.1672-3678.2006.03.004]
 ZHANG Tian-zhi,YANG Jong,YANG Shun-kai,et al.Study on the biotransformation of a new steroid substrate into C11β-hydroxylated product by Curvularia lunata AS3.4381[J].Chinese Journal of Bioprocess Engineering,2006,4(03):22-27.[doi:10.3969/j.issn.1672-3678.2006.03.004]
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新月弯孢霉AS 3.4381对新型甾体底物C11β-羟基化()
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
4
期数:
2006年03期
页码:
22-27
栏目:
出版日期:
2006-08-30

文章信息/Info

Title:
Study on the biotransformation of a new steroid substrate into C11β-hydroxylated product by Curvularia lunata AS3.4381
作者:
张天智1杨炯1杨顺楷2杨亚力2肖勇2
1. 四川大学, 药学院, 成都, 610041; 2. 中国科学院, 成都生物研究所, 成都, 610041
Author(s):
ZHANG Tian-zhi1 YANG Jong1 YANG Shun-kai2 YANG Ya-li2 XIAO Yong2
关键词:
新月弯孢霉生物转化底物(16α17β-二甲基-17-丙酰基雄甾-14-二烯-3-酮)Rimexolone(11β-羟基-16α17β-二甲基-17-丙酰基雄甾-14-二烯-3-酮)
分类号:
Q93.97
DOI:
10.3969/j.issn.1672-3678.2006.03.004
摘要:
应用本实验室保藏的新月弯孢霉Curvularia lunata AS 3.4381对新型甾体化合物(Ⅰ)(16α,17β-二甲基-17-丙酰基雄甾-1,4-二烯-3-酮)作为底物进行生物转化C11β-羟基化反应的研究.实验研究结果表明,采用Ⅱ级发酵的工艺,收获新月弯孢霉菌丝体作为生物催化剂,在磷酸缓冲液介质体系中,对化合物Ⅰ的C11位实现β羟基化,生成皮质激素药物.测试数据TLC,MS,IR及1H NMR证明了该产物的化学结构,表明生物转化产物为C11β-羟基-16α,17β-二甲基-17-丙酰基雄甾-1,4-二烯-3-酮.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:中国科学院知识创新工程项目
更新日期/Last Update: 1900-01-01