|本期目录/Table of Contents|

[1]吴永宏,肖明芳,吕浩,等.核酸酶P1催化水解热变性DNA的研究[J].生物加工过程,2005,3(02):53-57.[doi:10.3969/j.issn.1672-3678.2005.02.012]
　WU Yong-Hong,XIAO Ming-fang,LU Hao,et al.Studies on enzymatic hydrolysis of the heat-denatured DNA by nuclease P1[J].Chinese Journal of Bioprocess Engineering,2005,3(02):53-57.[doi:10.3969/j.issn.1672-3678.2005.02.012]
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核酸酶P1催化水解热变性DNA的研究()
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
3
期数:
2005年02期
页码:
53-57
栏目:
出版日期:
2005-05-30

文章信息/Info

Title:
Studies on enzymatic hydrolysis of the heat-denatured DNA by nuclease P1
作者:
吴永宏; 肖明芳; 吕浩; 应汉杰
南京工业大学, 制药与生命科学学院, 南京, 210009
Author(s):
WU Yong-Hong;  XIAO Ming-fang;  LU Hao;  YING Han-jie
关键词:
核酸酶P1; 水解; 热变性DNA; 优化; 米氏常数
分类号:
Q814.9
DOI:
10.3969/j.issn.1672-3678.2005.02.012
摘要:
用桔青霉菌株IM02(Penicillium citrinum IM02)发酵制得的核酸酶 P1催化水解热变性DNA,在研究温度、底物浓度、pH、酶加入量等因素对水解结果的影响的实验基础上,通过正交实验获得最佳的工艺条件:温度58 ℃,pH 6.5,DNA质量浓度4.0 g/L,酶加入量4%,脱氧核苷酸的得率92%.在此基础上推导出其催化米氏常数Km=5.818×10-2 g/L.

参考文献/References:

[1] Fujimoto M, Kuninaka A, Yoshino H. Identity of Phosphodiesterase and Phosphomonoesterase Activities with Nuclease P1 (a Nucleasefrom Penicillium citrinum) [J]. Agricultural and Biological Chemistry, 1974.785-790.
[2] Fujimoto M, Kuninaka A, Yoshino H. Substrate Specificity of Nuclease P1 [J]. Agricultural and Biological Chemistry, 1974.1555-1561.
[3] Fujimoto M, Fujiyama K, Kuninaka A. Mode of Action of Nuclease P1 on Nucleic Acids and Its Specificity for SyntheticPhosphodiesters [J]. Agricultural and Biological Chemistry, 1974.2141-2147.
[4] Kaufman HE, Heidelberger CL. Therapeutic antiviral action of 5-trifluoromethyl-2′-deoxyuridine in herpes simplex keratitis [J]. Science, 1964, (3632):585-586.doi:10.1126/science.145.3632.585.
[5] 唐孝礼, 周永红. 鱼精DNA的快速无污染提取工艺 [J]. 广州化工, 1996(4):21-23.
[6] 李建武, 萧能庚, 余瑞元. 生物化学实验原理和方法 [M]. 北京:北京大学出版社, 1994.174-176:270-275.

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备注/Memo

备注/Memo:
基金项目：教育部跨世纪优秀人才培养计划，江苏省333新世纪科学技术带头人培养工程
更新日期/Last Update: 1900-01-01