|本期目录/Table of Contents|

[1]韩伟,马婧,胡贤卿,等.桑椹中黄酮类物质的大孔树脂纯化工艺[J].生物加工过程,2010,8(04):27-32.[doi:10.3969/j.issn.1672-3678.2010.04.006]
 HAN Wei,MA Jing,HU Xianqing,et al.Purification of mulberry total flavonoids by macroporous resins[J].Chinese Journal of Bioprocess Engineering,2010,8(04):27-32.[doi:10.3969/j.issn.1672-3678.2010.04.006]
点击复制

桑椹中黄酮类物质的大孔树脂纯化工艺(/HTML)
分享到:

《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
8
期数:
2010年04期
页码:
27-32
栏目:
出版日期:
2010-07-31

文章信息/Info

Title:
Purification of mulberry total flavonoids by macroporous resins
作者:
韩伟马婧胡贤卿罗晓颖
华东理工大学中药现代化工程中心,上海200237
Author(s):
HAN WeiMA JingHU XianqingLUO Xiaoying
Engineering Center for Traditional Chinese Medicine Modernization, East China University of Science
and Technology, Shanghai 200237, China
关键词:
大孔吸附树脂桑椹纯化
分类号:
R284.2
DOI:
10.3969/j.issn.1672-3678.2010.04.006
文献标志码:
A
摘要:
以桑椹中黄酮类物质的吸附量和解吸率为指标,对比分析HZ-801、HZ-816、HZ-818等12种大孔吸附树脂对桑椹提取液的分离纯化效果,优选出最佳树脂HZ-801并通过对上样液pH、上样液质量浓度、上样量、吸附流速、洗脱剂质量浓度、洗脱剂用量、洗脱流速等影响因素的考察,确定最优工艺:吸附阶段上样液pH=4,上样液质量浓度0.45 mg/mL,上样量420 mL,吸附流速120 mL/h,动态吸附量(干树脂)25.34 mg/g,吸附率84.25%;洗脱阶段的洗脱剂体积分数为60%乙醇,洗脱剂用量270 mL,洗脱流速120 mL/h.此优化工艺条件下的洗脱率为85.78%,总黄酮纯度从23.64%提高到82.36%.

相似文献/References:

[1]董惠钧,赵军杰,陈启林,等.大孔吸附树脂结合硅胶柱层析纯化环孢菌素A[J].生物加工过程,2010,8(03):6.[doi:10.3969/j.issn.1672-3678.2010.03.002]
 DONG Hui-jun,ZHAO Jun-jie,CHEN Qi-lin,et al.Purification of cyclosporin A by using macroporous resin and silica gel column chromatography[J].Chinese Journal of Bioprocess Engineering,2010,8(04):6.[doi:10.3969/j.issn.1672-3678.2010.03.002]
[2]傅珊,万文倩,杨文革,等.油橄榄叶中橄榄苦苷的分离纯化[J].生物加工过程,2011,9(04):31.[doi:10.3969/j.issn.1672-3678.2011.04.007]
 FU Shan,WAN Wenqian,YANG Wenge,et al.Separation and purification of oleuropein from olive leaves[J].Chinese Journal of Bioprocess Engineering,2011,9(04):31.[doi:10.3969/j.issn.1672-3678.2011.04.007]

更新日期/Last Update: