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[1]常 军,胡 娜,张铁军,等.响应面法优化獐牙菜苦苷生物转化条件[J].生物加工过程,2010,8(01):28-33.[doi:doi:10.3969/j.issn.1672-3678.2010.01.006]
 CHANG Jun,HU Na,ZHANG Tie-jun,et al.Biotransformation optimization of swertiamarin by response surface methodology[J].Chinese Journal of Bioprocess Engineering,2010,8(01):28-33.[doi:doi:10.3969/j.issn.1672-3678.2010.01.006]
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响应面法优化獐牙菜苦苷生物转化条件
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
8
期数:
2010年01期
页码:
28-33
栏目:
出版日期:
2010-01-30

文章信息/Info

Title:
Biotransformation optimization of swertiamarin by response surface methodology
文章编号:
1672-3678(2010)01-0028-06
作者:
常 军1 胡 娜1 张铁军2刘昌孝2田成旺2
1.江西科技师范学院 南昌市天然产物与活性物质重点实验室, 南昌 330013;
2.天津药物研究院, 天津 300193)
Author(s):
CHANG Jun1 HU Na1 ZHANG Tie-jun2 LIU Chang-xiao2 TIAN Cheng-wang2
1.Nanchang Key Laboratory of Natural Product and Bioactive Materials, Jiangxi Science and Technology Normal University,Nanchang 330013, China;
2.Tianjin Institute of Pharmaceutical Research, Tianjin 300193, China
关键词:
獐牙菜苦苷 生物转化 獐牙菜新碱 红百金花内酯
分类号:
TQ920.1
DOI:
doi:10.3969/j.issn.1672-3678.2010.01.006
文献标志码:
A
摘要:
采用响应面法对獐牙菜苦苷的生物转化条件进行优化,首先应用Plackett-Burman方法筛选出对转化具有显著性影响的因素, 再用中心响应面法的Response Optimizer工具对最优转化条件进行了分析和预测, 最后对预测结果进行了验证。结果表明, 獐牙菜新碱的最优化转化条件(mg/mL)为MgSO4·7H2O 5.14、MnSO4·4H2O 3.42、葡萄糖9.95、蛋白胨9.24、pH 5.80。在此条件下獐牙菜新碱的最大转化产率为17.64%; 红百金花内酯的最优转化条件(mg/mL)为MgSO4·7H2O 4.09、MnSO4·4H2O 6.07、葡萄糖7.37、酵母膏7.37, pH 5.73。在此条件下红百金花内酯的最大转化产率为8.81%。


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备注/Memo

备注/Memo:
收稿日期:2009-06-08
基金项目:天津市应用基础重点资助项目(08jczDJc24700); 江西科技师范学院博士基金资助项目
作者简介: 常 军(1974—),男,湖北监利人,博士,讲师,研究方向:生物制药,E-mail:changjun8772@163.com
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