|本期目录/Table of Contents|

[1]钟传圣,饶志明,夏海锋,等.Mycobacterium sp.高效转化植物甾醇为雄甾-1,4-二烯-3,17-二酮的诱变选育[J].生物加工过程,2009,7(03):43-46.
 ZHONG Chuan-sheng,RAO Zhi-ming,XIA Hai-feng,et al.Mutation breeding of Mycobacterium sp. for transformation of phytosterol into androst-1,4-diene-3,17-dione[J].Chinese Journal of Bioprocess Engineering,2009,7(03):43-46.
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Mycobacterium sp.高效转化植物甾醇为雄甾-1,4-二烯-3,17-二酮的诱变选育
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
7
期数:
2009年03期
页码:
43-46
栏目:
出版日期:
2009-05-30

文章信息/Info

Title:
Mutation breeding of Mycobacterium sp. for transformation of phytosterol into androst-1,4-diene-3,17-dione
文章编号:
1672-3678(2009)03-0043-04
作者:
钟传圣1饶志明1夏海锋1许正宏2方慧英1诸葛健1
1. 江南大学 工业生物技术教育部重点实验室,无锡214122;
2. 江南大学 医药学院,无锡214122
Author(s):
ZHONG Chuan-sheng 1 RAO Zhi-ming 1 XIA Hai-feng1 XU Zheng-hong2FANG Hui-ying1 ZHUGE Jian1
1. Key Laboratory of Industrial Biotechnology of the Ministry of Education, Jiangnan University, Wuxi 214122, China;
2. School of Medicine and Pharmaceutics, Jiangnan University, Wuxi 214122, China
关键词:
紫外诱变植物甾醇微生物转化雄甾-14-二烯-317-二酮
分类号:
Q815
文献标志码:
A
摘要:
采用紫外线、亚硝基胍复合诱变雄甾-4-烯-3,17-二酮(AD)和雄甾-1,4-二烯-3,17-二酮(ADD)的转化产生菌Mycobacterium sp.,结合平板筛选,获得一株遗传性状稳定单产ADD的突变菌株Mycobacterium sp.-11,其ADD质量浓度达到1 246 mg/L,比原始菌株(484 mg/L)提高了150%,经初步优化后发酵液中ADD最高达到1 430 mg/L,发酵液中ADD质量占ADD、AD两产物质量总和的比例由70%提高到99.1%。

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2008-09-11
基金项目:国家重点基础研究发展计划(973计划)资助项目(2007CB707804);国家高技术研究发展计划(863 计划)资助项目(2006AA020103);国家自然科学基金资助项目(20676053);教育部新世纪优秀人才支持计划资助项目(NCET-07-0380);江苏省青年科技创新人才(学术带头人)基金资助项目 (BK2006504)。
作者简介:钟传圣(1985—),男,江西永丰人,硕士研究生,研究方向:微生物学;饶志明(联系人),教授,E-mail:raozm@yahoo.com.cn
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