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[1]张智平,倪国荣,赖崇德,等.大孔吸附树脂吸附链霉菌702抗真菌[J].生物加工过程,2009,7(02):23-28.
 ZHANG Zhi-ping,NI Guo-rong,LAI Chong-de,et al.Macroporous resin in adsorption process of antifungal produced by Streptomyces 702[J].Chinese Journal of Bioprocess Engineering,2009,7(02):23-28.
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大孔吸附树脂吸附链霉菌702抗真菌
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
7
期数:
2009年02期
页码:
23-28
栏目:
出版日期:
2009-03-30

文章信息/Info

Title:
Macroporous resin in adsorption process of antifungal produced by Streptomyces 702
文章编号:
1672-3678(2009)02-0023-06
作者:
张智平倪国荣赖崇德涂国全
江西农业大学 生物科学与工程学院,南昌330045
Author(s):
ZHANG Zhi-pingNI Guo-rongLAI Chong-deTU Guo-quan
Department of Bioscience and Bioengineering,Jiangxi Agricultural University,Nanchang 330045,China
关键词:
抗真菌大孔树脂链霉菌
分类号:
TQ424.3
文献标志码:
A
摘要:
研究大孔吸附树脂吸附链霉菌702抗真菌活性物质的工艺条件。采用5种不同大孔吸附树脂吸附链霉菌702发酵液提取液中抗真菌活性物质,选择吸附效果较佳树脂进行吸附条件优化,以桔青霉为指示菌,纳他霉素为对照抗生素,采用“管碟法”测定抗真菌活性物质含量。结果发现,XAD18树脂吸附效果较好,获得优化吸附条件:上样液pH 6,NaCl质量浓度10 g/L,上样量22.63 mg/g湿树脂,吸附流速2.5 mL/min,水洗体积180 mL,洗脱流速1.5 mL/min,洗脱剂为体积分数10%、50%和90%的甲醇,洗脱方式为梯度洗脱。在确定的工艺条件下XAD18对链霉菌702抗真菌活性物质的吸附率可达90%,洗脱率可达75%,回收率可达80%。

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2008-08-22
基金项目:江西省科技支撑计划项目(赣科发计字(2007)196号);广东省科技攻关项目(2006B130010040)
作者简介:张智平(1983—),男,江西永丰人,硕士研究生,研究方向:微生物发酵工程;涂国全(联系人),教授,E-mail:tuguoquan@263.com
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