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[1]蔡谦,吴明火,郑裕国,等.对羟基苯乙腈水合酶高产菌株的选育[J].生物加工过程,2005,3(03):38-41.[doi:10.3969/j.issn.1672-3678.2005.03.008]
　CAI Qian,WU Ming-huo,ZHENG Yu-guo,et al.Isolation of strains highly yielding p-hydroxyphenylacetamide nitrile hydratase[J].Chinese Journal of Bioprocess Engineering,2005,3(03):38-41.[doi:10.3969/j.issn.1672-3678.2005.03.008]
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对羟基苯乙腈水合酶高产菌株的选育()
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
3
期数:
2005年03期
页码:
38-41
栏目:
出版日期:
2005-08-30

文章信息/Info

Title:
Isolation of strains highly yielding p-hydroxyphenylacetamide nitrile hydratase
作者:
蔡谦; 吴明火; 郑裕国; 沈寅初
浙江工业大学, 生物与环境工程学院, 杭州, 310032
Author(s):
CAI Qian;  WU Ming-huo;  ZHENG Yu-guo;  SHEN Yin-chu
关键词:
对羟基苯乙酰胺(HPAD); 对羟基苯乙腈; 腈水合酶
分类号:
Q933
DOI:
10.3969/j.issn.1672-3678.2005.03.008
摘要:
从全省各处采集的50多份土样筛选到一株产腈水合酶能力较高的菌株E10a,该菌株产生的腈水合酶为非诱导酶,产酶条件优化实验结果表明,产酶培养基组成:麦芽糖20 g/L,酵母膏5 g/L,尿素7.5 g/L,味精0.75 g/L,K2HPO4 0.5 g/L,KH2PO4 0.5 g/L,MgSO4 0.5 g/L,FeSO4·7H2O 10 mg/L,CoCl2 10 mg/L,微量元素母液0.8 ml/L;最佳培养条件为:培养温度28 ℃,摇床转速150 r/min,培养基起始pH 7.0,培养时间5 d,在优化培养条件下,1 h可将1 g/L质量浓度的底物对羟基苯乙腈全部转化为对羟基苯乙酰胺.

参考文献/References:

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相似文献/References:

[1]吴明火,蔡谦,郑裕国,等.对羟基苯乙腈水解酶产生菌的筛选及产酶条件研究[J].生物加工过程,2005,3(04):32.[doi:10.3969/j.issn.1672-3678.2005.04.007]
　WU Ming-huo,CAI Qian,ZHENG Yu-guo,et al.Screening for p-hydroxyphenylcyanide nitrilase and study of enzyme production conditions[J].Chinese Journal of Bioprocess Engineering,2005,3(03):32.[doi:10.3969/j.issn.1672-3678.2005.04.007]

备注/Memo

备注/Memo:
基金项目：国家自然科学基金
更新日期/Last Update: 1900-01-01